027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Human MET ELISA Kit
MET (GeneName), Hepatocyte growth factor receptor (ProteinName), MET_HUMAN.
Product Name:

Human MET/ Hepatocyte growth factor receptor ELISA Kit

Cat.#:

E9559h

Brand:
EIAab®
Regulatory Status:
Alternative:

HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
34pg/mL
Specificity:
Natural and recombinant human Hepatocyte growth factor receptor
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Signal Transduction
Human MET ELISA Kit
Size & Price: Sign In.
×
Human MET ELISA Kit
Email *
Message *
Please Sign In.
Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Recovery
Recovery was determined by spiking various levels of Hepatocyte growth factor receptor into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

94

88-100

Plasma

96

90-102

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hepatocyte growth factor receptor and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

105-115%

103-113%

99-111%

103-112%

EDTA plasma(n=5)

83-93%

109-118%

94-104%

99-108%

heparin plasma(n=5)

87-97%

 

84-93%

99-109%

108-119%

 

General Annotation


Sub Unit:
Heterodimer made of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1. Interacts when phosphorylated with downstream effectors including STAT3, PIK3R1, SRC, PCLG1, GRB2 and GAB1. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. Interacts with GRB10. Interacts with PTPN1 and PTPN2. Interacts with LECT2; this interaction may have an antagonistic effect on receptor activation (PubMed:27334921). Interacts with HSP90AA1 and HSP90AB1; the interaction suppresses MET kinase activity (PubMed:26517842).


Function:
Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.


Subcellular Location:
Isoform 3 Secreted



[1].
"A mutation of MET, encoding hepatocyte growth factor receptor, is associated with human DFNB97 hearing loss."

[2].
"Ethnic differences and functional analysis of MET mutations in lung cancer."

[3].
"Met gene copy number predicts the prognosis for completely resected non-small cell lung cancer."

[4].
"Novel splice variants derived from the receptor tyrosine kinase superfamily are potential therapeutics for rheumatoid arthritis."

[5].
"A genetic variant that disrupts MET transcription is associated with autism."
Close
Sample Data
Close
Sample Data
Close
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name *
Email *
Message: *
Inform
new Message
Size Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Size Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com