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Home  >  ELISA Kit  >  Mouse Metrnl ELISA Kit
Metrnl (GeneName), Meteorin-like protein (ProteinName), METRL_MOUSE.
Product Name:

Mouse Metrnl/ Meteorin-like protein ELISA Kit

Cat.#:

E7391m

Brand:
EIAab®
Regulatory Status:
Alternative:

Subfatin

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant mouse Meteorin-like protein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Mouse Metrnl ELISA Kit
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Mouse Metrnl ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.9%

Recovery
Recovery was determined by spiking various levels of Meteorin-like protein into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

88

82-94

Plasma

90

84-96

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Meteorin-like protein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-120%

120-120%

102-110%

108-118%

EDTA plasma(n=5)

102-113%

95-108%

100-109%

91-101%

heparin plasma(n=5)

107-115%

 

106-116%

105-115%

102-112%

 

General Annotation


Sub Unit:
N/A


Function:
Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures.


Subcellular Location:
Secreted


This product has not yet been referenced specifically in any publications.

[1].
"Subfatin is a novel adipokine and unlike Meteorin in adipose and brain expression."

[2].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[3].
"METRNL decreases during adipogenesis and inhibits adipocyte differentiation leading to adipocyte hypertrophy in humans."

[4].
"Architecture of the human interactome defines protein communities and disease networks."

[5].
"Meteorin-Like Shows Unique Expression Pattern in Bone and Its Overexpression Inhibits Osteoblast Differentiation."

[6].
"Adipocyte Metrnl Antagonizes Insulin Resistance Through PPARγ Signaling."

[7].
"A human interactome in three quantitative dimensions organized by stoichiometries and abundances."

[8].
"Meteorin-like is a hormone that regulates immune-adipose interactions to increase beige fat thermogenesis."

[9].
"Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT)."

[10].
"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
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