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MAT2A (GeneName), S-adenosylmethionine synthase isoform type-2 (ProteinName), METK2_HUMAN.
Product Name:

Human MAT2A/ S-adenosylmethionine synthase isoform type-2 ELISA Kit

Cat.#:

E12626h

Brand:
EIAab®
Regulatory Status:
Alternative:

AdoMet synthase 2, Methionine adenosyltransferase 2, MAT 2, Methionine adenosyltransferase II, MAT-II, AMS2, MATA2

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.15ng/ml
Specificity:
Natural and recombinant human S-adenosylmethionine synthase isoform type-2
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Human MAT2A ELISA Kit
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Human MAT2A ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.3%

Recovery
Recovery was determined by spiking various levels of S-adenosylmethionine synthase isoform type-2 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

106

100-112

Plasma

108

102-114

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S-adenosylmethionine synthase isoform type-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

109-118%

104-104%

98-107%

93-103%

EDTA plasma(n=5)

108-118%

106-116%

114-114%

92-102%

heparin plasma(n=5)

92-105%

 

87-99%

98-107%

99-99%

 

General Annotation


Sub Unit:
Heterotrimer; composed of a catalytic MAT2A homodimer that binds one regulatory MAT2B chain (PubMed:10644686, PubMed:23189196). Heterohexamer; composed of a central, catalytic MAT2A homotetramer flanked on either side by a regulatory MAT2B chain (PubMed:25075345).


Function:
Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.


Subcellular Location:
N/A


This product has not yet been referenced specifically in any publications.

[1].
"Role of methionine adenosyltransferase 2A and S-adenosylmethionine in mitogen-induced growth of human colon cancer cells."

[2].
"Cloning, expression, and functional characterization of the beta regulatory subunit of human methionine adenosyltransferase (MAT II)."

[3].
"Cloning and functional characterization of the 5'-flanking region of human methionine adenosyltransferase 2A gene."

[4].
"Molecular cloning and developmental expression of a human kidney S-adenosylmethionine synthetase."

[5].
"The U6 snRNA m6A Methyltransferase METTL16 Regulates SAM Synthetase Intron Retention."

[6].
"Crystallography captures catalytic steps in human methionine adenosyltransferase enzymes."

[7].
"Structure and function study of the complex that synthesizes S-adenosylmethionine."

[8].
"Insight into S-adenosylmethionine biosynthesis from the crystal structures of the human methionine adenosyltransferase catalytic and regulatory subunits."

[9].
"A candidate gene study of folate-associated one carbon metabolism genes and colorectal cancer risk."

[10].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."
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