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MAT1A (GeneName), S-adenosylmethionine synthase isoform type-1 (ProteinName), METK1_HUMAN.
Product Name:

Human MAT1A/ S-adenosylmethionine synthase isoform type-1 ELISA Kit

Cat.#:

E0246h

Brand:
EIAab®
Regulatory Status:
Alternative:

AdoMet synthase 1, Methionine adenosyltransferase 1, MAT 1, Methionine adenosyltransferase I/III, MAT-I/III, AMS1, MATA1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.1ng/mL
Specificity:
Natural and recombinant human S-adenosylmethionine synthase isoform type-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human MAT1A ELISA Kit
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Human MAT1A ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Recovery
Recovery was determined by spiking various levels of S-adenosylmethionine synthase isoform type-1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S-adenosylmethionine synthase isoform type-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-107%

101-111%

83-93%

85-95%

EDTA plasma(n=5)

109-119%

93-103%

105-117%

108-118%

heparin plasma(n=5)

104-115%

 

87-98%

90-99%

90-102%

 

General Annotation


Sub Unit:
Homotetramer (MAT-I); dimer of dimers (PubMed:23425511). Homodimer (MAT-III).


Function:
Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.


Subcellular Location:
N/A


This product has not yet been referenced specifically in any publications.

[1].
"Demyelination of the brain is associated with methionine adenosyltransferase I/III deficiency."

[2].
"MAT1A variants are associated with hypertension, stroke, and markers of DNA damage and are modulated by plasma vitamin B-6 and folate."

[3].
"Methionine adenosyltransferase I/III deficiency: novel mutations and clinical variations."

[4].
"Dominant inheritance of isolated hypermethioninemia is associated with a mutation in the human methionine adenosyltransferase 1A gene."

[5].
"Molecular mechanisms of an inborn error of methionine pathway. Methionine adenosyltransferase deficiency."

[6].
"Characterization of a full-length cDNA encoding human liver S-adenosylmethionine synthetase: tissue-specific gene expression and mRNA levels in hepatopathies."

[7].
"Insight into S-adenosylmethionine biosynthesis from the crystal structures of the human methionine adenosyltransferase catalytic and regulatory subunits."

[8].
"Human variation in alcohol response is influenced by variation in neuronal signaling genes."

[9].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

[10].
"Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia."
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