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Home  >  ELISA Kit  >  Human MTR ELISA Kit
MTR (GeneName), Methionine synthase (ProteinName), METH_HUMAN.
Product Name:

Human MTR/ Methionine synthase ELISA Kit

Cat.#:

E14988h

Brand:
EIAab®
Regulatory Status:
Alternative:

5-methyltetrahydrofolate--homocysteine methyltransferase, Vitamin-B12 dependent methionine synthase, MS

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.625-40ng/mL
Sensitivity:
0.23ng/ml
Specificity:
Natural and recombinant human Methionine synthase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human MTR ELISA Kit
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Human MTR ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.2%

Recovery
Recovery was determined by spiking various levels of Methionine synthase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Methionine synthase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-108%

81-91%

101-111%

89-99%

EDTA plasma(n=5)

88-98%

111-123%

112-120%

106-116%

heparin plasma(n=5)

86-96%

 

80-88%

101-111%

81-91%

 

General Annotation


Sub Unit:
N/A


Function:
Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Analysis of methionine synthase reductase polymorphisms for neural tube defects risk association."

[2].
"Cloning, mapping and RNA analysis of the human methionine synthase gene."

[3].
"5-Methyltetrahydrofolate-homocysteine methyltransferase gene polymorphism (MTR) and risk of head and neck cancer."

[4].
"[Folate gene polymorphism and the risk of Down syndrome pregnancies in young Chinese women]."

[5].
"MTR polymorphic variant A2756G and retinoblastoma risk in Brazilian children."

[6].
"The effects of polymorphisms in methylenetetrahydrofolate reductase (MTHFR), methionine synthase (MTR), and methionine synthase reductase (MTRR) on the risk of cervical intraepithelial neoplasia and cervical cancer in Korean women."

[7].
"Genetic polymorphisms involved in folate metabolism and concentrations of methylmalonic acid and folate on plasma homocysteine and risk of coronary artery disease."

[8].
"DNA promoter methylation in breast tumors: no association with genetic polymorphisms in MTHFR and MTR."

[9].
"Genetic variants of methyl metabolizing enzymes and epigenetic regulators: associations with promoter CpG island hypermethylation in colorectal cancer."

[10].
"Effect of common polymorphisms in folate uptake and metabolism genes on frequency of micronucleated lymphocytes in a South Australian cohort."
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