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Home  >  ELISA Kit  >  Rat Mertk ELISA Kit
Mertk (GeneName), Tyrosine-protein kinase Mer (ProteinName), MERTK_RAT.
Product Name:

Rat Mertk/ Tyrosine-protein kinase Mer ELISA Kit

Cat.#:

E1419r

Brand:
EIAab®
Regulatory Status:
Alternative:

Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, Mer

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.083ng/mL
Specificity:
Natural and recombinant rat Tyrosine-protein kinase Mer
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Neurosciences
Rat Mertk ELISA Kit
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Rat Mertk ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.4%

Recovery
Recovery was determined by spiking various levels of Tyrosine-protein kinase Mer into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

115

109-120

Plasma

117

111-120

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tyrosine-protein kinase Mer and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

108-120%

84-94%

91-104%

108-117%

EDTA plasma(n=5)

107-120%

81-93%

91-100%

102-112%

heparin plasma(n=5)

91-100%

 

81-92%

94-104%

106-117%

 

General Annotation


Sub Unit:
Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6. Interacts with VAV1 in a phosphotyrosine-independent manner.


Function:
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.


Subcellular Location:
Membrane Single-pass type I membrane protein


This product has not yet been referenced specifically in any publications.

[1].
"MERTK polymorphisms associated with risk of haematological disorders among Korean SLE patients."

[2].
"An SH2 domain-dependent, phosphotyrosine-independent interaction between Vav1 and the Mer receptor tyrosine kinase: a mechanism for localizing guanine nucleotide-exchange factor action."

[3].
"Mutations in MERTK, the human orthologue of the RCS rat retinal dystrophy gene, cause retinitis pigmentosa."

[4].
"Cloning and mRNA expression analysis of a novel human protooncogene, c-mer."

[5].
"Galectin-3 is a new MerTK-specific eat-me signal."
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