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MEPE (GeneName), Matrix extracellular phosphoglycoprotein (ProteinName), MEPE_HUMAN.
Product Name:

Human MEPE/ Matrix extracellular phosphoglycoprotein ELISA Kit

Cat.#:

E1232h

Brand:
EIAab®
Regulatory Status:
Alternative:

Osteoblast/osteocyte factor 45, OF45, Osteoregulin

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.94-60ng/mL
Sensitivity:
0.43ng/mL
Specificity:
Natural and recombinant human Matrix extracellular phosphoglycoprotein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human MEPE ELISA Kit
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Human MEPE ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Recovery
Recovery was determined by spiking various levels of Matrix extracellular phosphoglycoprotein into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

92

86-98

Plasma

94

88-100

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix extracellular phosphoglycoprotein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

80-89%

108-120%

101-111%

93-103%

EDTA plasma(n=5)

104-113%

110-122%

100-110%

99-109%

heparin plasma(n=5)

93-102%

 

109-119%

81-91%

102-111%

 

General Annotation


Sub Unit:
Interacts (via the ASARM motif) with PHEX; the interaction is zinc-dependent.


Function:
Promotes renal phosphate excretion and inhibits intestinal phosphate absorption (PubMed:14962809, PubMed:19005008). Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes (PubMed:18162525, PubMed:19998030, PubMed:22766095). Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (By similarity). Promotes dental pulp stem cell proliferation and differentiation (PubMed:22341070).


Subcellular Location:
Secreted Extracellular space Extracellular matrix


This product has not yet been referenced specifically in any publications.

[1].
"MEPE, a new gene expressed in bone marrow and tumors causing osteomalacia."

[2].
"MEPE's diverse effects on mineralization."

[3].
"Changes in matrix extracellular phosphoglycoprotein expression before and during in vitro osteogenic differentiation of human dental papilla mesenchymal cells."

[4].
"Matrix extracellular phosphoglycoprotein inhibits phosphate transport."

[5].
"Matrix extracellular phosphoglycoprotein (MEPE) is highly expressed in osteocytes in human bone."

[6].
"MEPE/OF45, a new dentin/bone matrix protein and candidate gene for dentin diseases mapping to chromosome 4q21."

[7].
"Mepe, the gene encoding a tumor-secreted protein in oncogenic hypophosphatemic osteomalacia, is expressed in bone."

[8].
"The effect of matrix extracellular phosphoglycoprotein and its downstream osteogenesis-related gene expression on the proliferation and differentiation of human dental pulp cells."

[9].
"Secreted kinase phosphorylates extracellular proteins that regulate biomineralization."

[10].
"Genetic determinants of cardiovascular events among women with migraine: a genome-wide association study."
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