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Home  >  ELISA Kit  >  Rat Mep1a ELISA Kit
Mep1a (GeneName), Meprin A subunit alpha (ProteinName), MEP1A_RAT.
Product Name:

Rat Mep1a/ Meprin A subunit alpha ELISA Kit

Cat.#:

E2226r

Brand:
EIAab®
Regulatory Status:
Alternative:

Endopeptidase-2, Endopeptidase-24.18 subunit alpha, E-24.18, MEP-1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant rat Meprin A subunit alpha
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Rat Mep1a ELISA Kit
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Rat Mep1a ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.6%

Recovery
Recovery was determined by spiking various levels of Meprin A subunit alpha into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

104

98-110

Plasma

106

100-112

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Meprin A subunit alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-107%

92-102%

112-122%

104-114%

EDTA plasma(n=5)

103-113%

100-109%

110-120%

97-106%

heparin plasma(n=5)

103-113%

 

102-111%

105-113%

111-119%

 

General Annotation


Sub Unit:
Homotetramer consisting of disulfide-linked alpha subunits, homooligomer consisting of disulfide-linked alpha subunit homodimers, or heterotetramer of two alpha and two beta subunits formed by non-covalent association of two disulfide-linked heterodimers (By similarity). Interacts with MBL2 through its carbohydrate moiety. This interaction may inhibit its catalytic activity.


Function:
N/A


Subcellular Location:
Membrane Single-pass type I membrane protein


This product has not yet been referenced specifically in any publications.

[1].
"MEP1A allele for meprin A metalloprotease is a susceptibility gene for inflammatory bowel disease."

[2].
"The structural genes, MEP1A and MEP1B, for the alpha and beta subunits of the metalloendopeptidase meprin map to human chromosomes 6p and 18q, respectively."

[3].
"Cloning of the PABA peptide hydrolase alpha subunit (PPH alpha) from human small intestine and its expression in COS-1 cells."

[4].
"Exclusion of the juvenile myoclonic epilepsy gene EFHC1 as the cause of migraine on chromosome 6, but association to two rare polymorphisms in MEP1A and RHAG."

[5].
"Structure and expression of the human MEP1A gene encoding the alpha subunit of metalloendopeptidase meprin A."

[6].
"Polarised expression of human intestinal N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase (human meprin) alpha and beta subunits in Madin-Darby canine kidney cells."

[7].
"Metalloproteinase meprin α regulates migration and invasion of human hepatocarcinoma cells and is a mediator of the oncoprotein Reptin."

[8].
"Meprin metalloproteases: Molecular regulation and function in inflammation and fibrosis."

[9].
"Metalloproteases meprin-ɑ (MEP1A) is a prognostic biomarker and promotes proliferation and invasion of colorectal cancer."

[10].
"Dependence of Human Colorectal Cells Lacking the FBW7 Tumor Suppressor on the Spindle Assembly Checkpoint."
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