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Home  >  ELISA Kit  >  Human MLPH ELISA Kit
MLPH (GeneName), Melanophilin (ProteinName), MELPH_HUMAN.
Product Name:

Human MLPH/ Melanophilin ELISA Kit

Cat.#:

E13463h

Brand:
EIAab®
Regulatory Status:
Alternative:

Exophilin-3, Slp homolog lacking C2 domains a, SlaC2-a, Synaptotagmin-like protein 2a, SLAC2A

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/ml
Sensitivity:
39pg/mL
Specificity:
Natural and recombinant human Melanophilin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human MLPH ELISA Kit
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Human MLPH ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.7%

Recovery
Recovery was determined by spiking various levels of Melanophilin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

107

101-113

Plasma

109

103-115

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Melanophilin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

84-94%

104-114%

81-92%

103-112%

EDTA plasma(n=5)

108-117%

103-111%

99-109%

95-105%

heparin plasma(n=5)

102-112%

 

106-117%

107-115%

101-110%

 

General Annotation


Sub Unit:
Binds RAB27A that has been activated by GTP-binding via its N-terminus. Binds MYO5A via its C-terminal coiled coil domain.


Function:
Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Genome-wide association study identifies new prostate cancer susceptibility loci."

[2].
"High throughput SNP and expression analyses of candidate genes for non-syndromic oral clefts."

[3].
"In vitro reconstitution of a transport complex containing Rab27a, melanophilin and myosin Va."

[4].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[5].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[6].
"Rab27b is up-regulated in human Griscelli syndrome type II melanocytes and linked to the actin cytoskeleton via exon F-Myosin Va transcripts."

[7].
"Interactions of human Myosin Va isoforms, endogenously expressed in human melanocytes, are tightly regulated by the tail domain."

[8].
"Griscelli syndrome restricted to hypopigmentation results from a melanophilin defect (GS3) or a MYO5A F-exon deletion (GS1)."

[9].
"Characterization of the molecular defects in Rab27a, caused by RAB27A missense mutations found in patients with Griscelli syndrome."

[10].
"A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport."
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