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MELK (GeneName), Maternal embryonic leucine zipper kinase (ProteinName), MELK_HUMAN.
Product Name:

Human MELK/ Maternal embryonic leucine zipper kinase ELISA Kit

Cat.#:

E15058h

Brand:
EIAab®
Regulatory Status:
Alternative:

hMELK, Protein kinase Eg3, pEg3 kinase, Protein kinase PK38, hPK38, Tyrosine-protein kinase MELK, KIAA0175

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.098ng/ml
Specificity:
Natural and recombinant human Maternal embryonic leucine zipper kinase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cancer
Human MELK ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.4%

Recovery
Recovery was determined by spiking various levels of Maternal embryonic leucine zipper kinase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

86

80-92

Plasma

88

82-94

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Maternal embryonic leucine zipper kinase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

83-93%

84-94%

89-100%

104-116%

EDTA plasma(n=5)

107-117%

113-123%

87-97%

100-110%

heparin plasma(n=5)

95-105%

 

108-118%

106-116%

100-109%

 

General Annotation


Sub Unit:
Monomer. Interacts with ZNF622 and PPP1R8.


Function:
Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.


Subcellular Location:
Cell membrane Peripheral membrane protein


This product has not yet been referenced specifically in any publications.

[1].
"Dysregulated expression of Fau and MELK is associated with poor prognosis in breast cancer."

[2].
"Maternal embryonic leucine zipper kinase is a key regulator of the proliferation of malignant brain tumors, including brain tumor stem cells."

[3].
"Maternal embryonic leucine zipper kinase transcript abundance correlates with malignancy grade in human astrocytomas."

[4].
"Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family."

[5].
"CDC25B phosphorylated by pEg3 localizes to the centrosome and the spindle poles at mitosis."

[6].
"Substrate specificity and activity regulation of protein kinase MELK."

[7].
"Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1."

[8].
"Human pEg3 kinase associates with and phosphorylates CDC25B phosphatase: a potential role for pEg3 in cell cycle regulation."

[9].
"Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1."

[10].
"Siomycin A targets brain tumor stem cells partially through a MELK-mediated pathway."
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