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Mecp2 (GeneName), Methyl-CpG-binding protein 2 (ProteinName), MECP2_RAT.
Product Name:

Rat Mecp2/ Methyl-CpG-binding protein 2 ELISA Kit

Cat.#:

E15238r

Brand:
EIAab®
Regulatory Status:
Alternative:

MeCp-2 protein

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78.0 pg/mL -5000 pg/mL
Sensitivity:
36 pg/mL
Specificity:
Natural and recombinant rat Methyl-CpG-binding protein 2
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Neurosciences
Rat Mecp2 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.4%

Recovery
Recovery was determined by spiking various levels of Methyl-CpG-binding protein 2 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Methyl-CpG-binding protein 2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

93-103%

108-118%

81-91%

104-116%

EDTA plasma(n=5)

91-103%

102-112%

87-97%

80-93%

heparin plasma(n=5)

97-107%

 

102-112%

104-114%

101-112%

 

General Annotation


Sub Unit:
Interacts with FNBP3 (By similarity). Interacts with CDKL5 (By similarity). Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells (PubMed:17296936). Interacts with NCOR2.


Function:
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).


Subcellular Location:
Nucleus Colocalized with methyl-CpG in the genome.


This product has not yet been referenced specifically in any publications.

[1].
"Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2."

[2].
"[Analysis of the parental origin of MECP2 mutations in patients with Rett syndrome]."

[3].
"High frequency of nonrecurrent MECP2 duplications among Brazilian males with mental retardation."

[4].
"[Methyl-CpG-binding protein 2 gene and CDKL5 gene mutation in patients with Rett syndrome: analysis of 177 Chinese pediatric patients]."

[5].
"Variants within MECP2, a key transcription regulator, are associated with increased susceptibility to lupus and differential gene expression in patients with systemic lupus erythematosus."

[6].
"Spectrum of MECP2 mutations in New Zealand Rett syndrome patients."

[7].
"MECP2 mutations in Malaysian Rett syndrome patients."

[8].
"Structural variation in Xq28: MECP2 duplications in 1% of patients with unexplained XLMR and in 2% of male patients with severe encephalopathy."

[9].
"Methyl-CpG-binding protein 2 polymorphisms and vulnerability to autism."

[10].
"Drug-resistant epilepsy and epileptic phenotype-EEG association in MECP2 mutated Rett syndrome."
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