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Home  >  ELISA Kit  >  Rat Mdh2 ELISA Kit
Mdh2 (GeneName), Malate dehydrogenase, mitochondrial (ProteinName), MDHM_RAT.
Product Name:

Rat Mdh2/ Malate dehydrogenase, mitochondrial ELISA Kit

Cat.#:

E0548r

Brand:
EIAab®
Regulatory Status:
Alternative:

Mor1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.066ng/mL
Specificity:
Natural and recombinant rat Malate dehydrogenase, mitochondrial
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Epigenetics
Rat Mdh2 ELISA Kit
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Rat Mdh2 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Recovery
Recovery was determined by spiking various levels of Malate dehydrogenase, mitochondrial into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

95

89-101

Plasma

97

91-103

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Malate dehydrogenase, mitochondrial and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

82-90%

110-120%

108-120%

109-117%

EDTA plasma(n=5)

101-112%

97-107%

93-105%

89-99%

heparin plasma(n=5)

98-108%

 

102-111%

101-111%

94-104%

 

General Annotation


Sub Unit:
Homodimer.


Function:
N/A


Subcellular Location:
Mitochondrion matrix


This product has not yet been referenced specifically in any publications.

[1].
"Mutations in MDH2, Encoding a Krebs Cycle Enzyme, Cause Early-Onset Severe Encephalopathy."

[2].
"Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score."

[3].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

[4].
"Regulation of cellular metabolism by protein lysine acetylation."

[5].
"L-2-hydroxyglutaric aciduria, a defect of metabolite repair."

[6].
"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."

[7].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[8].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[9].
"The DNA sequence of human chromosome 7."

[10].
"Tom20-mediated mitochondrial protein import in muscle cells during differentiation."
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