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Mdh1 (GeneName), Malate dehydrogenase, cytoplasmic (ProteinName), MDHC_RAT.
Product Name:

Rat Mdh1/ Malate dehydrogenase, cytoplasmic ELISA Kit

Cat.#:

E2124r

Brand:
EIAab®
Regulatory Status:
Alternative:

Cytosolic malate dehydrogenase, Mdh

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.183ng/mL
Specificity:
Natural and recombinant rat Malate dehydrogenase, cytoplasmic
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Metabolism
Rat Mdh1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Recovery
Recovery was determined by spiking various levels of Malate dehydrogenase, cytoplasmic into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

90

84-96

Plasma

92

86-98

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Malate dehydrogenase, cytoplasmic and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

98-106%

96-106%

103-112%

105-115%

EDTA plasma(n=5)

105-115%

104-113%

107-117%

102-112%

heparin plasma(n=5)

88-98%

 

95-105%

102-112%

90-99%

 

General Annotation


Sub Unit:
Homodimer.


Function:
N/A


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Molecular cloning and mapping of a human cDNA for cytosolic malate dehydrogenase (MDH1)."

[2].
"Acetylation of malate dehydrogenase 1 promotes adipogenic differentiation via activating its enzymatic activity."

[3].
"Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?"

[4].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

[5].
"Physiogenomic comparison of edema and BMI in patients receiving rosiglitazone or pioglitazone."

[6].
"A nucleocytoplasmic malate dehydrogenase regulates p53 transcriptional activity in response to metabolic stress."

[7].
"Ultra high throughput sequencing excludes MDH1 as candidate gene for RP28-linked retinitis pigmentosa."

[8].
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."

[9].
"Developmental regulation and cellular distribution of human cytosolic malate dehydrogenase (MDH1)."

[10].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
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