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MAD2L1 (GeneName), Mitotic spindle assembly checkpoint protein MAD2A (ProteinName), MD2L1_HUMAN.
Product Name:

Human MAD2L1/ Mitotic spindle assembly checkpoint protein MAD2A ELISA Kit

Cat.#:
-
Brand:
EIAab®
Regulatory Status:
Alternative:

HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2

Detection Method:
ELISA
Specificity:
Natural and recombinant human Mitotic spindle assembly checkpoint protein MAD2A
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Research Area:
Cancer
Human MAD2L1 ELISA Kit
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Product Datasheets


General Annotation


Sub Unit:
Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR. Binds to UBD during mitosis. Interacts with isoform 1 and isoform 2 of NEK2. Interacts with HSF1; this interaction occurs in mitosis (PubMed:18794143).


Function:
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.


Subcellular Location:
Nucleus Chromosome Centromere Kinetochore Cytoplasm Cytoplasm Cytoskeleton Spindle pole Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.


This product has not yet been referenced specifically in any publications.

[1].
"Identification of a human mitotic checkpoint gene: hsMAD2."

[2].
"Perturbation of the chromosomal binding of RCC1, Mad2 and survivin causes spindle assembly defects and mitotic catastrophe."

[3].
"Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimer."

[4].
"The Mad2 conformational dimer: structure and implications for the spindle assembly checkpoint."

[5].
"p31comet blocks Mad2 activation through structural mimicry."

[6].
"The Mad2 spindle checkpoint protein has two distinct natively folded states."

[7].
"Mad2 phosphorylation regulates its association with Mad1 and the APC/C."

[8].
"The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20."

[9].
"Crystal structure of the tetrameric Mad1-Mad2 core complex: implications of a 'safety belt' binding mechanism for the spindle checkpoint."

[10].
"Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta."
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