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Mcts1 (GeneName), Malignant T-cell-amplified sequence 1 (ProteinName), MCTS1_RAT.
Product Name:

Rat Mcts1/ Malignant T-cell-amplified sequence 1 ELISA Kit

Cat.#:

E15021r

Brand:
EIAab®
Regulatory Status:
Alternative:

MCT-1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.059ng/mL
Specificity:
Natural and recombinant rat Malignant T-cell-amplified sequence 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Rat Mcts1 ELISA Kit
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Rat Mcts1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Recovery
Recovery was determined by spiking various levels of Malignant T-cell-amplified sequence 1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

84

80-90

Plasma

86

80-92

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Malignant T-cell-amplified sequence 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-116%

97-107%

82-93%

86-96%

EDTA plasma(n=5)

94-104%

90-102%

103-113%

84-96%

heparin plasma(n=5)

96-106%

 

91-101%

92-102%

104-114%

 

General Annotation


Sub Unit:
Interacts (via PUA domain) with DENR.


Function:
Anti-oncogene that plays a role in cell cycle regulation; decreases cell doubling time and anchorage-dependent growth; shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Plays a role as translation enhancer; Recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway; increased gamma-irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when overexpressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines overexpressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell; Positively regulates phosphorylation of MAPK1 and MAPK3.


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"MCT-1 protein interacts with the cap complex and modulates messenger RNA translational profiles."

[2].
"A novel candidate oncogene, MCT-1, is involved in cell cycle progression."

[3].
"DENR-MCT-1 promotes translation re-initiation downstream of uORFs to control tissue growth."

[4].
"Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling."

[5].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[6].
"Expression of the candidate MCT-1 oncogene in B- and T-cell lymphoid malignancies."

[7].
"Expression and stabilization of the MCT-1 protein by DNA damaging agents."

[8].
"Architecture of the human interactome defines protein communities and disease networks."

[9].
"Structural and Functional Insights into Human Re-initiation Complexes."

[10].
"Crystal Structure of the Human Ribosome in Complex with DENR-MCT-1."
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