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GJA1 (基因名), Gap junction alpha-1 protein (蛋白名), CXA1_BOVIN.

产品名称:

Bovine GJA1/ Gap junction alpha-1 protein ELISA Kit
间隙连接蛋白α-1

货号:

E2241b

商标:

EIAab^®

监管等级:

RUO

别名:

Connexin-43, Vascular smooth muscle connexin-43, Cx43

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

78-5000pg/mL

灵敏度:

18pg/mL

特异性:

Natural and recombinant bovine Gap junction alpha-1 protein

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献 ( 4 )
序列 / 3D
常见问题解答
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通用注释

亚单元:

A connexon is composed of a hexamer of connexins. Interacts with SGSM3 (By similarity). Interacts with RIC1/CIP150 (By similarity). Interacts with CNST and CSNK1D (By similarity). Interacts (via C-terminus) with TJP1. Interacts (via C-terminus) with SRC (via SH3 domain). Interacts (not ubiquitinated) with UBQLN4 (via UBA domain) (By similarity). Interacts with NOV. Interacts with TMEM65.

功能:

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles.

亚细胞位置:

Cell membrane Multi-pass membrane protein Cell junction Gap junction Endoplasmic reticulum Localizes at the intercalated disk (ICD) in cardiomyocytes and proper localization at ICD is dependent on TMEM65.

数据库链接

UniGene:

Bt.15972

SMR:

P18246

STRING:

9913.ENSBTAP00000002398

KEGG:

bta:281193

Pfam:

PF00029

Uniprot:

P18246

相关产品

Human GJA1 ELISA Kit

Mouse Gja1 ELISA Kit

Rat Gja1 ELISA Kit

Chicken GJA1 ELISA Kit

Pig GJA1 ELISA Kit

序列长度:

383

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

383:

GHELQPLAIV | DQRPSSRASS | RASSRPRPDD | LEI

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Do chlorinated insecticides (aldrin and DDT) or products of their transformations (dieldrin and DDE) impair signal transfer from regulators (oxytocin and relaxin) of bovine myometrium motility in vitro?"

M H Wrobel.

From: Environmental Research

[2].

"Chloroorganic (DDT) and organophosphate (malathion) insecticides impair the motor function of the bovine cervix"

M.H. Wrobel, J. Mlynarczuk.

From: Toxicology and Applied Pharmacology

[3].

"Do commonly used herbicides (atrazine and glyphosate) have the potential to impair the contractions, prostaglandin releasing and conducting of oxytocin signal at the bovine cervix in vitro?"

M H Wróbel, J Młynarczuk, R Rękawiecki, et al.

From: Theriogenology

[4].

W H Wrobel.

From: Environmental Research

[1].

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒可以做多少个样本？

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒使用视频？

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛间隙连接蛋白α-1(GJA1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛间隙连接蛋白α-1(GJA1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛间隙连接蛋白α-1(GJA1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛间隙连接蛋白α-1(GJA1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Bovine GJA1/ Gap junction alpha-1 protein ELISA Kit 间隙连接蛋白α-1

E2241b

Connexin-43, Vascular smooth muscle connexin-43, Cx43

Bovine GJA1/ Gap junction alpha-1 protein ELISA Kit
间隙连接蛋白α-1