027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Human ASPA ELISA Kit
ASPA (GeneName), Aspartoacylase (ProteinName), ACY2_HUMAN.
Product Name:

Human ASPA/ Aspartoacylase ELISA Kit

Cat.#:

E2260h

Brand:
EIAab®
Regulatory Status:
Alternative:

Aminoacylase-2, ACY-2, ACY2, ASP

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
39.8pg/mL
Specificity:
Natural and recombinant human Aspartoacylase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Neurosciences
Human ASPA ELISA Kit
Size & Price: Sign In.
×
Human ASPA ELISA Kit
Email *
Message *
Please Sign In.
Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery
Recovery was determined by spiking various levels of Aspartoacylase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

90

84-96

Plasma

92

86-98

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aspartoacylase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

90-100%

82-92%

109-119%

100-110%

EDTA plasma(n=5)

96-106%

111-119%

108-118%

89-99%

heparin plasma(n=5)

109-118%

 

100-110%

93-105%

92-100%

 

General Annotation


Sub Unit:
Homodimer.


Function:
Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids.


Subcellular Location:
Cytoplasm Nucleus


This product has not yet been referenced specifically in any publications.

[1].
"Structure of aspartoacylase, the brain enzyme impaired in Canavan disease."

[2].
"Cloning of the human aspartoacylase cDNA and a common missense mutation in Canavan disease."

[3].
"Examination of the mechanism of human brain aspartoacylase through the binding of an intermediate analogue."

[4].
"Identification of the zinc binding ligands and the catalytic residue in human aspartoacylase, an enzyme involved in Canavan disease."

[5].
"Purification and preliminary characterization of brain aspartoacylase."

[6].
"Two novel aspartoacylase gene (ASPA) missense mutations specific to Norwegian and Swedish patients with Canavan disease."

[7].
"Identification and characterization of novel mutations of the aspartoacylase gene in non-Jewish patients with Canavan disease."

[8].
"Identification and expression of eight novel mutations among non-Jewish patients with Canavan disease."

[9].
"The molecular basis of canavan (aspartoacylase deficiency) disease in European non-Jewish patients."

[10].
"Canavan disease: mutations among Jewish and non-Jewish patients."
Close
Sample Data
Close
Sample Data
Close
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name *
Email *
Message: *
Inform
new Message
Size Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Size Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com