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ACY1 (GeneName), Aminoacylase-1 (ProteinName), ACY1_HUMAN.
Product Name:

Human ACY1/ Aminoacylase-1 ELISA Kit

Cat.#:

E2259h

Brand:
EIAab®
Regulatory Status:
Alternative:

ACY-1, N-acyl-L-amino-acid amidohydrolase

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.791ng/mL
Specificity:
Natural and recombinant human Aminoacylase-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Human ACY1 ELISA Kit
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Human ACY1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.2%

Recovery
Recovery was determined by spiking various levels of Aminoacylase-1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

85

80-91

Plasma

87

81-93

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aminoacylase-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

112-120%

106-116%

108-117%

107-116%

EDTA plasma(n=5)

117-117%

93-103%

105-113%

102-112%

heparin plasma(n=5)

111-119%

 

96-105%

108-118%

89-99%

 

General Annotation


Sub Unit:
Homodimer. Interacts with SPHK1.


Function:
Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Neurological findings in aminoacylase 1 deficiency."

[2].
"Mutations in ACY1, the gene encoding aminoacylase 1, cause a novel inborn error of metabolism."

[3].
"Aminoacylase I deficiency: a novel inborn error of metabolism."

[4].
"Essential roles of zinc ligation and enzyme dimerization for catalysis in the aminoacylase-1/M20 family."

[5].
"The nucleotide sequence of human aminoacylase-1."

[6].
"Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer."

[7].
"Human aminoacylase-1: cloning, regional assignment to distal chromosome 3p21.1, and identification of a cross-hybridizing sequence on chromosome 18."

[8].
"The molecular basis of aminoacylase 1 deficiency."

[9].
"Probing the acyl-binding pocket of aminoacylase-1."

[10].
"NMR spectroscopy of aminoacylase 1 deficiency, a novel inborn error of metabolism."
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