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ACTA1 (GeneName), Actin, alpha skeletal muscle (ProteinName), ACTS_HUMAN.
Product Name:

Human ACTA1/ Actin, alpha skeletal muscle ELISA Kit

Cat.#:

E1634h

Brand:
EIAab®
Regulatory Status:
Alternative:

Alpha-actin-1, ACTA

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.27 ng/mL
Specificity:
Natural and recombinant human Actin, alpha skeletal muscle
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human ACTA1 ELISA Kit
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Human ACTA1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery
Recovery was determined by spiking various levels of Actin, alpha skeletal muscle into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

83

80-89

Plasma

85

80-91

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Actin, alpha skeletal muscle and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

87-96%

107-116%

102-113%

97-106%

EDTA plasma(n=5)

86-95%

95-105%

91-101%

104-117%

heparin plasma(n=5)

89-99%

 

82-93%

110-120%

105-115%

 

General Annotation


Sub Unit:
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (By similarity). Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells.


Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.


Subcellular Location:
Cytoplasm Cytoskeleton


This product has not yet been referenced specifically in any publications.

[1].
"The pathogenesis of ACTA1-related congenital fiber type disproportion."

[2].
"The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins."

[3].
"Autosomal dominant nemaline myopathy with intranuclear rods due to mutation of the skeletal muscle ACTA1 gene: clinical and pathological variability within a kindred."

[4].
"Actin mutations are one cause of congenital fibre type disproportion."

[5].
"Follow-up of nemaline myopathy in two patients with novel mutations in the skeletal muscle alpha-actin gene (ACTA1)."

[6].
"Heterogeneity of nemaline myopathy cases with skeletal muscle alpha-actin gene mutations."

[7].
"Evidence for a dominant-negative effect in ACTA1 nemaline myopathy caused by abnormal folding, aggregation and altered polymerization of mutant actin isoforms."

[8].
"Nemaline myopathy caused by mutations in the muscle alpha-skeletal-actin gene."

[9].
"Mild phenotype of nemaline myopathy with sleep hypoventilation due to a mutation in the skeletal muscle alpha-actin (ACTA1) gene."

[10].
"Mutations in the skeletal muscle alpha-actin gene in patients with actin myopathy and nemaline myopathy."
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