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Home  >  ELISA Kit  >  Human ACTN4 ELISA Kit
ACTN4 (GeneName), Alpha-actinin-4 (ProteinName), ACTN4_HUMAN.
Product Name:

Human ACTN4/ Alpha-actinin-4 ELISA Kit

Cat.#:

E1460h

Brand:
EIAab®
Regulatory Status:
Alternative:

Non-muscle alpha-actinin 4

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/ml
Sensitivity:
32pg/mL
Specificity:
Natural and recombinant human Alpha-actinin-4
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Epigenetics
Human ACTN4 ELISA Kit
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Human ACTN4 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery
Recovery was determined by spiking various levels of Alpha-actinin-4 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-actinin-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

103-113%

109-119%

115-124%

87-99%

EDTA plasma(n=5)

94-104%

105-117%

89-98%

99-109%

heparin plasma(n=5)

104-113%

 

89-99%

109-117%

94-104%

 

General Annotation


Sub Unit:
Homodimer; antiparallel (By similarity). Binds TRIM3 at the N-terminus (By similarity). Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation (By similarity). Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 (By similarity). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with BAIAP1 and PDLIM2. Interacts with PPARG and RARA. Binds to VCL; this interaction triggers VCL conformational changes (PubMed:15988023).


Function:
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778).


Subcellular Location:
Nucleus Cytoplasm Cell junction Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC-2, and bladder cancer KU-7).


This product has not yet been referenced specifically in any publications.

[1].
"Mutations in ACTN4, encoding alpha-actinin-4, cause familial focal segmental glomerulosclerosis."

[2].
"ACTN4 gene mutations and single nucleotide polymorphisms in idiopathic focal segmental glomerulosclerosis."

[3].
"Association analysis of podocyte slit diaphragm genes as candidates for diabetic nephropathy."

[4].
"Crystal structure of the actin-binding domain of alpha-actinin-4 Lys255Glu mutant implicated in focal segmental glomerulosclerosis."

[5].
"Familial focal segmental glomerulosclerosis associated with an ACTN4 mutation and paternal germline mosaicism."
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