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ACTN3 (GeneName), Alpha-actinin-3 (ProteinName), ACTN3_HUMAN.
Product Name:

Human ACTN3/ Alpha-actinin-3 ELISA Kit

Cat.#:

E1104h

Brand:
EIAab®
Regulatory Status:
Alternative:

Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500pg/mL
Sensitivity:
4.1pg/mL
Specificity:
Natural and recombinant human Alpha-actinin-3
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cancer
Human ACTN3 ELISA Kit
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Human ACTN3 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Recovery
Recovery was determined by spiking various levels of Alpha-actinin-3 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

100

94-106

Plasma

102

96-108

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-actinin-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-120%

105-115%

96-104%

100-112%

EDTA plasma(n=5)

95-104%

96-106%

109-119%

111-123%

heparin plasma(n=5)

110-119%

 

108-118%

102-111%

97-109%

 

General Annotation


Sub Unit:
Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1.


Function:
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.


Subcellular Location:
N/A


This product has not yet been referenced specifically in any publications.

[1].
"Cloning and characterization of two human skeletal muscle alpha-actinin genes located on chromosomes 1 and 11."

[2].
"ACTN3 and ACE genotypes in elite Jamaican and US sprinters."

[3].
"Strength, power, fiber types, and mRNA expression in trained men and women with different ACTN3 R577X genotypes."

[4].
"Association between the ACTN3 R577X polymorphism and artistic gymnastic performance in Italy."

[5].
"ACTN3 R577X polymorphism and Israeli top-level athletes."

[6].
"alpha-actinin-3 and performance."

[7].
"Endurance performance: genes or gene combinations?"

[8].
"Human angiotensin-converting enzyme I/D and alpha-actinin 3 R577X genotypes and muscle functional and contractile properties."

[9].
"Is there an ACE ID - ACTN3 R577X polymorphisms interaction that influences sprint performance?"

[10].
"Association of the ACTN3 R577X polymorphism with power athlete status in Russians."
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