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首页  >  酶联免疫试剂盒  >  Human ACTN1 ELISA Kit
ACTN1 (基因名), Alpha-actinin-1 (蛋白名), ACTN1_HUMAN.
产品名称:

Human ACTN1/ Alpha-actinin-1 ELISA Kit
Alpha-actinin-1

货号:

E0376h

商标:
EIAab®
监管等级:
别名:

Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/ml
灵敏度:
0.078ng/mL
特异性:
Natural and recombinant human Alpha-actinin-1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Human ACTN1 ELISA Kit
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Human ACTN1 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤5.6%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.8%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

91

85-97

Plasma

93

87-99

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-118%

106-116%

102-113%

108-120%

EDTA plasma(n=5)

101-111%

96-108%

99-109%

81-90%

heparin plasma(n=5)

101-110%

 

101-111%

89-99%

93-102%

 

通用注释


亚单元:
Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP. Interacts with PSD. Interacts with MICALL2 (By similarity). Interacts with DNM2 and CTTN.


功能:
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.


亚细胞位置:
Cytoplasm Cytoskeleton Cytoplasm Myofibril Sarcomere Z line Cell membrane Cell junction Cell projection Ruffle Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures (By similarity).


该产品尚未在任何出版物中被引用。

[1].
"Postsynaptic recruitment of Dendrin depends on both dendritic mRNA transport and synaptic anchoring."

[2].
"Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene."

[3].
"A missense mutation in the alpha-actinin 1 gene (ACTN1) is the cause of autosomal dominant macrothrombocytopenia in a large French family."

[4].
"ACTN1 mutations cause congenital macrothrombocytopenia."

[5].
"Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells."

[6].
"Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia."

[7].
"Genome-wide and candidate gene association study of cigarette smoking behaviors."

[8].
"Isoform-specific contributions of alpha-actinin to glioma cell mechanobiology."

[9].
"Cathepsin X cleaves the beta2 cytoplasmic tail of LFA-1 inducing the intermediate affinity form of LFA-1 and alpha-actinin-1 binding."

[10].
"Mactinin, a fragment of cytoskeletal alpha-actinin, is a novel inducer of heat shock protein (Hsp)-90 mediated monocyte activation."
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