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ACTN1 (GeneName), Alpha-actinin-1 (ProteinName), ACTN1_HUMAN.
Product Name:

Human ACTN1/ Alpha-actinin-1 ELISA Kit

Cat.#:

E0376h

Brand:
EIAab®
Regulatory Status:
Alternative:

Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/ml
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant human Alpha-actinin-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cancer
Human ACTN1 ELISA Kit
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Human ACTN1 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Recovery
Recovery was determined by spiking various levels of Alpha-actinin-1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

91

85-97

Plasma

93

87-99

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-actinin-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-118%

106-116%

102-113%

108-120%

EDTA plasma(n=5)

101-111%

96-108%

99-109%

81-90%

heparin plasma(n=5)

101-110%

 

101-111%

89-99%

93-102%

 

General Annotation


Sub Unit:
Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP. Interacts with PSD. Interacts with MICALL2 (By similarity). Interacts with DNM2 and CTTN.


Function:
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.


Subcellular Location:
Cytoplasm Cytoskeleton Cytoplasm Myofibril Sarcomere Z line Cell membrane Cell junction Cell projection Ruffle Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures (By similarity).


This product has not yet been referenced specifically in any publications.

[1].
"Postsynaptic recruitment of Dendrin depends on both dendritic mRNA transport and synaptic anchoring."

[2].
"Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene."

[3].
"A missense mutation in the alpha-actinin 1 gene (ACTN1) is the cause of autosomal dominant macrothrombocytopenia in a large French family."

[4].
"ACTN1 mutations cause congenital macrothrombocytopenia."

[5].
"Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells."

[6].
"Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia."

[7].
"Genome-wide and candidate gene association study of cigarette smoking behaviors."

[8].
"Isoform-specific contributions of alpha-actinin to glioma cell mechanobiology."

[9].
"Cathepsin X cleaves the beta2 cytoplasmic tail of LFA-1 inducing the intermediate affinity form of LFA-1 and alpha-actinin-1 binding."

[10].
"Mactinin, a fragment of cytoskeletal alpha-actinin, is a novel inducer of heat shock protein (Hsp)-90 mediated monocyte activation."
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