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Home  >  ELISA Kit  >  Rat Actb ELISA Kit
Actb (GeneName), Actin, cytoplasmic 1 (ProteinName), ACTB_RAT.
Product Name:

Rat Actb/ Actin, cytoplasmic 1 ELISA Kit

Cat.#:

E8725r

Brand:
EIAab®
Regulatory Status:
Alternative:

Beta-actin

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.172ng/mL
Specificity:
Natural and recombinant rat Actin, cytoplasmic 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Signal Transduction
Rat Actb ELISA Kit
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Rat Actb ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.9%

Recovery
Recovery was determined by spiking various levels of Actin, cytoplasmic 1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

101

95-107

Plasma

103

97-109

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Actin, cytoplasmic 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

102-112%

108-117%

107-119%

91-99%

EDTA plasma(n=5)

102-114%

98-107%

89-101%

110-120%

heparin plasma(n=5)

105-114%

 

96-106%

106-115%

108-120%

 

General Annotation


Sub Unit:
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCSAM. Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Interacts with TBC1D21.


Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.


Subcellular Location:
Cytoplasm Cytoskeleton Localized in cytoplasmic mRNP granules containing untranslated mRNAs.


This product has not yet been referenced specifically in any publications.

[1].
"A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma."

[2].
"De novo mutations in the actin genes ACTB and ACTG1 cause Baraitser-Winter syndrome."

[3].
"Nuclear ErbB2 enhances translation and cell growth by activating transcription of ribosomal RNA genes."

[4].
"Tumor suppressor down-regulated in renal cell carcinoma 1 (DRR1) is a stress-induced actin bundling factor that modulates synaptic efficacy and cognition."

[5].
"[Establishment of a multiplex ligation-dependent SNP genotyping method and its application in the detection of genes related to chemotherapeutic drugs in breast cancer]."

[6].
"Molecular composition of IMP1 ribonucleoprotein granules."

[7].
"HGAL, a lymphoma prognostic biomarker, interacts with the cytoskeleton and mediates the effects of IL-6 on cell migration."

[8].
"A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia."

[9].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[10].
"Activation of the GLI oncogene through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms: pericytoma with t(7;12)."
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