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首页  >  酶联免疫试剂盒  >  Human ACTB ELISA Kit
ACTB (基因名), Actin, cytoplasmic 1 (蛋白名), ACTB_HUMAN.
产品名称:

Human ACTB/ Actin, cytoplasmic 1 ELISA Kit
细胞质肌动蛋白1

货号:

E8725h

商标:
EIAab®
监管等级:
别名:

Beta-actin

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.312-20ng/mL
灵敏度:
0.18 ng/mL
特异性:
Natural and recombinant human Actin, cytoplasmic 1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Signal Transduction
Human ACTB ELISA Kit
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Human ACTB ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤6.0%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤9.1%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

100

94-106

Plasma

102

96-108

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

103-113%

80-90%

99-109%

95-105%

EDTA plasma(n=5)

111-120%

101-111%

98-107%

98-108%

heparin plasma(n=5)

80-80%

 

95-105%

95-106%

99-108%

 

通用注释


亚单元:
Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCSAM. Interacts with TBC1D21.


功能:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.


亚细胞位置:
Cytoplasm Cytoskeleton Localized in cytoplasmic mRNP granules containing untranslated mRNAs.


该产品尚未在任何出版物中被引用。

[1].
"A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma."

[2].
"De novo mutations in the actin genes ACTB and ACTG1 cause Baraitser-Winter syndrome."

[3].
"Nuclear ErbB2 enhances translation and cell growth by activating transcription of ribosomal RNA genes."

[4].
"Tumor suppressor down-regulated in renal cell carcinoma 1 (DRR1) is a stress-induced actin bundling factor that modulates synaptic efficacy and cognition."

[5].
"[Establishment of a multiplex ligation-dependent SNP genotyping method and its application in the detection of genes related to chemotherapeutic drugs in breast cancer]."

[6].
"Molecular composition of IMP1 ribonucleoprotein granules."

[7].
"HGAL, a lymphoma prognostic biomarker, interacts with the cytoskeleton and mediates the effects of IL-6 on cell migration."

[8].
"A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia."

[9].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[10].
"Activation of the GLI oncogene through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms: pericytoma with t(7;12)."
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