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Home  >  ELISA Kit  >  Human ACTA2 ELISA Kit
ACTA2 (GeneName), Actin, aortic smooth muscle (ProteinName), ACTA_HUMAN.
Product Name:

Human ACTA2/ Actin, aortic smooth muscle ELISA Kit

Cat.#:

E1342h

Brand:
EIAab®
Regulatory Status:
Alternative:

Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTSA, ACTVS, GIG46

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200ng/mL
Sensitivity:
1.56ng/mL
Specificity:
Natural and recombinant human Actin, aortic smooth muscle
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human ACTA2 ELISA Kit
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Human ACTA2 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.2%

Recovery
Recovery was determined by spiking various levels of Actin, aortic smooth muscle into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Actin, aortic smooth muscle and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

94-104%

94-103%

95-104%

108-118%

EDTA plasma(n=5)

97-108%

95-105%

107-118%

111-120%

heparin plasma(n=5)

100-110%

 

97-107%

104-114%

88-100%

 

General Annotation


Sub Unit:
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.


Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.


Subcellular Location:
Cytoplasm Cytoskeleton


This product has not yet been referenced specifically in any publications.

[1].
"Mutations in smooth muscle alpha-actin (ACTA2) cause coronary artery disease, stroke, and Moyamoya disease, along with thoracic aortic disease."

[2].
"Mutations in smooth muscle alpha-actin (ACTA2) lead to thoracic aortic aneurysms and dissections."

[3].
"Risk of dissection in thoracic aneurysms associated with mutations of smooth muscle alpha-actin 2 (ACTA2)."

[4].
"Analysis of ACTA2 in European Moyamoya disease patients."

[5].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

[6].
"De novo ACTA2 mutation causes a novel syndrome of multisystemic smooth muscle dysfunction."

[7].
"Deciphering the impact of common genetic variation on lung cancer risk: a genome-wide association study."

[8].
"Mutation of ACTA2 gene as an important cause of familial and nonfamilial nonsyndromatic thoracic aortic aneurysm and/or dissection (TAAD)."

[9].
"A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease."

[10].
"Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection."
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