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ACSM1 (基因名), Acyl-coenzyme A synthetase ACSM1, mitochondrial (蛋白名), ACSM1_BOVIN.

产品名称:

Bovine ACSM1/ Acyl-coenzyme A synthetase ACSM1, mitochondrial ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

Acyl-CoA synthetase medium-chain family member 1, BUCS1, LAE, MACS1, Benzoate--CoA ligase, Butyrate--CoA ligase 1, Butyryl-coenzyme A synthetase 1, Lipoate-activating enzyme, Middle-chain acyl-CoA synthetase 1, XL-III, Xenobiotic/medium-chain fatty acid:CoA ligase XL-3, XM-ligase 3

检测方法:

ELISA

特异性:

Natural and recombinant bovine Acyl-coenzyme A synthetase ACSM1, mitochondrial

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格: 登录.

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Monomer.

功能:

Catalyzes the activation of fatty acids by CoA to produce an acyl-CoA, the first step in fatty acid metabolism (PubMed:11382754, PubMed:10561077). Capable of activating medium-chain fatty acids (e.g. butyric (C4) to decanoic (C10) acids), and certain carboxylate-containing xenobiotics, e.g. benzoate (PubMed:10561077, PubMed:11382754). Also catalyzes the activation of lipoate to lipoyl-nucleoside monophosphate (PubMed:11382754). Activates lipoate with GTP at a 1000-fold higher rate than with ATP and activates both (R)- and (S)-lipoate to the respective lipoyl-GMP, with a preference for (R)-lipoate (PubMed:11382754).

亚细胞位置:

Mitochondrion matrix Mitochondrion

数据库链接

UniGene:

Bt.64940

SMR:

Q9BEA2

STRING:

9913.ENSBTAP00000001859

KEGG:

bta:282576

Pfam:

PF00501

Uniprot:

Q9BEA2

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序列长度:

577

序列:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

586:

APYKYPRKVE | FVSELPKTIT | GKIKRSELRK | KEFGQK

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Molecular identification and characterization of two medium-chain acyl-CoA synthetases, MACS1 and the Sa gene product."

(PMID: 11470804)

[2].

"Two medium-chain acyl-coenzyme A synthetase genes, SAH and MACS1, are associated with plasma high-density lipoprotein cholesterol levels, but they are not associated with essential hypertension."

(PMID: 15361761)

[3].

"Gene variants associated with schizophrenia in a Norwegian genome-wide study are replicated in a large European cohort."

(PMID: 20185149)

[4].

"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

(PMID: 20628086)

[5].

"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

(PMID: 20877624)

[6].

"Genomewide association for schizophrenia in the CATIE study: results of stage 1."

(PMID: 18347602)

[7].

"15-prostaglandin dehydrogenase expression alone or in combination with ACSM1 defines a subgroup of the apocrine molecular subtype of breast carcinoma."

(PMID: 18632593)

[8].

"An acyl-CoA synthetase gene family in chromosome 16p12 may contribute to multiple risk factors."

(PMID: 12654705)

[9].

"Architecture of the human interactome defines protein communities and disease networks."

(PMID: 28514442)

[10].

"Genetic association of ACSM1 variation with schizophrenia and major depressive disorder in the Han Chinese population."

(PMID: 25656805)

[1].

牛ACSM1ELISA试剂盒可以做多少个样本？

牛ACSM1ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛ACSM1ELISA试剂盒使用视频？

牛ACSM1ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛ACSM1ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛ACSM1ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛ACSM1ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛ACSM1ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛ACSM1ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛ACSM1ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛ACSM1ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛ACSM1ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛ACSM1ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛ACSM1ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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