Aasdh (GeneName), Beta-alanine-activating enzyme (ProteinName), ACSF4_MOUSE.
Product Name:
Mouse Aasdh/ Beta-alanine-activating enzyme ELISA Kit
Cat.#:
E8952m
Brand:
EIAab®
Regulatory Status:
Alternative:
Protein LYS2 homolog, Acsf4, U26, Acyl-CoA synthetase family member 4
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.89ng/mL
Specificity:
Natural and recombinant mouse Beta-alanine-activating enzyme
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.8%
Recovery
Recovery was determined by spiking various levels of Beta-alanine-activating enzyme into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
104 |
98-110 |
|
Plasma |
106 |
100-112 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-alanine-activating enzyme and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
90-98% |
96-106% |
87-97% |
105-116% |
|
EDTA plasma(n=5) |
103-112% |
101-112% |
110-120% |
96-106% |
|
heparin plasma(n=5) |
93-103%
|
105-116% |
97-108% |
101-110% |
General Annotation
Sub Unit:
N/A
Function:
Covalently binds beta-alanine in an ATP-dependent manner to form a thioester bond with its phosphopantetheine group and transfers it to an as yet unknown acceptor via an amide bond. May be required for a post-translational protein modification or for post-transcriptional modification of an RNA.
Subcellular Location:
N/A
Database link
UniGene:
SMR:
STRING:
KEGG:
Pfam:
Uniprot:
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Cloning and characterization of a novel human homolog* of mouse U26, a putative PQQ-dependent AAS dehydrogenase."
[2].
"Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome."
[4].
"A Global Analysis of the Receptor Tyrosine Kinase-Protein Phosphatase Interactome."
[5].
"Architecture of the human interactome defines protein communities and disease networks."
[6].
"A human interactome in three quantitative dimensions organized by stoichiometries and abundances."
[7].
"Vertebrate Acyl?CoA synthetase family member?4 (ACSF4-U26) is a β-alanine-activating enzyme homologous to bacterial non-ribosomal peptide synthetase."
[8].
"Toward a comprehensive characterization of a human cancer cell phosphoproteome."
[9].
"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
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