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Aasdh (GeneName), Beta-alanine-activating enzyme (ProteinName), ACSF4_MOUSE.
Product Name:

Mouse Aasdh/ Beta-alanine-activating enzyme ELISA Kit

Cat.#:

E8952m

Brand:
EIAab®
Regulatory Status:
Alternative:

Protein LYS2 homolog, Acsf4, U26, Acyl-CoA synthetase family member 4

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.89ng/mL
Specificity:
Natural and recombinant mouse Beta-alanine-activating enzyme
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Mouse Aasdh ELISA Kit
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Mouse Aasdh ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.8%

Recovery
Recovery was determined by spiking various levels of Beta-alanine-activating enzyme into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

104

98-110

Plasma

106

100-112

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-alanine-activating enzyme and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

90-98%

96-106%

87-97%

105-116%

EDTA plasma(n=5)

103-112%

101-112%

110-120%

96-106%

heparin plasma(n=5)

93-103%

 

105-116%

97-108%

101-110%

 

General Annotation


Sub Unit:
N/A


Function:
Covalently binds beta-alanine in an ATP-dependent manner to form a thioester bond with its phosphopantetheine group and transfers it to an as yet unknown acceptor via an amide bond. May be required for a post-translational protein modification or for post-transcriptional modification of an RNA.


Subcellular Location:
N/A


This product has not yet been referenced specifically in any publications.

[1].
"Cloning and characterization of a novel human homolog* of mouse U26, a putative PQQ-dependent AAS dehydrogenase."

[2].
"Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome."

[3].
"Nutritional biochemistry: A new redox-cofactor vitamin for mammals."

[4].
"A Global Analysis of the Receptor Tyrosine Kinase-Protein Phosphatase Interactome."

[5].
"Architecture of the human interactome defines protein communities and disease networks."

[6].
"A human interactome in three quantitative dimensions organized by stoichiometries and abundances."

[7].
"Vertebrate Acyl?CoA synthetase family member?4 (ACSF4-U26) is a β-alanine-activating enzyme homologous to bacterial non-ribosomal peptide synthetase."

[8].
"Toward a comprehensive characterization of a human cancer cell phosphoproteome."

[9].
"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."

[10].
"The full-ORF clone resource of the German cDNA Consortium."
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