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Home  >  ELISA Kit  >  Human ACR ELISA Kit
ACR (GeneName), Acrosin (ProteinName), ACRO_HUMAN.
Product Name:

Human ACR/ Acrosin ELISA Kit

Cat.#:

E1947h

Brand:
EIAab®
Regulatory Status:
Alternative:

ACRS

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant human Acrosin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Human ACR ELISA Kit
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Human ACR ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.3%

Recovery
Recovery was determined by spiking various levels of Acrosin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

100

94-106

Plasma

102

96-108

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acrosin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

108-118%

107-117%

102-112%

91-101%

EDTA plasma(n=5)

105-114%

101-113%

104-116%

96-105%

heparin plasma(n=5)

102-114%

 

106-116%

98-107%

99-109%

 

General Annotation


Sub Unit:
Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5.


Function:
Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.


Subcellular Location:
N/A


This product has not yet been referenced specifically in any publications.

[1].
"Molecular cloning of human preproacrosin cDNA."

[2].
"Evaluation of the proacrosin/acrosin system and its mechanism of activation in human sperm extracts."

[3].
"Inhibition of acrosin by protein C inhibitor and localization of protein C inhibitor to spermatozoa."

[4].
"Molecular cloning, sequencing and restriction mapping of the genomic sequence encoding human proacrosin."

[5].
"Nucleotide sequence and exon-intron organization of the human proacrosin gene."

[6].
"Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC."

[7].
"Binding of recombinant human proacrosin/acrosin to zona pellucida glycoproteins. II. Participation of mannose residues in the interaction."

[8].
"A genome annotation-driven approach to cloning the human ORFeome."

[9].
"Biochemical and molecular studies of the proacrosin/acrosin system in patients with unexplained infertility."

[10].
"[The effect of semen antisperm antibody on human sperm acrosin activity]."
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