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Apeh (GeneName), Acylamino-acid-releasing enzyme (ProteinName), ACPH_RAT.
Product Name:

Rat Apeh/ Acylamino-acid-releasing enzyme ELISA Kit

Cat.#:

E14162r

Brand:
EIAab®
Regulatory Status:
Alternative:

AARE, Acyl-peptide hydrolase, APH, Acylaminoacyl-peptidase

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.163ng/mL
Specificity:
Natural and recombinant rat Acylamino-acid-releasing enzyme
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Epigenetics
Rat Apeh ELISA Kit
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Rat Apeh ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤4.8%

Recovery
Recovery was determined by spiking various levels of Acylamino-acid-releasing enzyme into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

99

93-105

Plasma

101

95-107

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acylamino-acid-releasing enzyme and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-114%

85-95%

100-112%

90-98%

EDTA plasma(n=5)

106-116%

89-99%

90-100%

101-111%

heparin plasma(n=5)

98-111%

 

91-91%

106-116%

85-95%

 

General Annotation


Sub Unit:
Homotetramer.


Function:
This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Identification of oxidized protein hydrolase of human erythrocytes as acylpeptide hydrolase."

[2].
"The nucleotide sequence of human acylamino acid-releasing enzyme."

[3].
"Crystallization and preliminary X-ray studies of human erythrocyte acylpeptide hydrolase."

[4].
"Acylpeptide hydrolase: inhibitors and some active site residues of the human enzyme."

[5].
"The gene from the short arm of chromosome 3, at D3F15S2, frequently deleted in renal cell carcinoma, encodes acylpeptide hydrolase."

[6].
"Genetic relationship between acylpeptide hydrolase and acylase, two hydrolytic enzymes with similar binding but different catalytic specificities."

[7].
"Meta-analysis identifies 29 additional ulcerative colitis risk loci, increasing the number of confirmed associations to 47."

[8].
"Association analysis of 3p21 with Crohn's disease in a New Zealand population."

[9].
"Acyl peptide hydrolase, a serine proteinase isolated from conditioned medium of neuroblastoma cells, degrades the amyloid-beta peptide."

[10].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
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