APEH (GeneName), Acylamino-acid-releasing enzyme (ProteinName), ACPH_HUMAN.
Product Name:
Human APEH/ Acylamino-acid-releasing enzyme ELISA Kit
Cat.#:
E14162h
Brand:
EIAab®
Regulatory Status:
Alternative:
AARE, Acyl-peptide hydrolase, APH, Acylaminoacyl-peptidase, Oxidized protein hydrolase, OPH, D3F15S2, D3S48E, DNF15S2
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.1ng/ml
Specificity:
Natural and recombinant human Acylamino-acid-releasing enzyme
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Epigenetics
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.1%
Recovery
Recovery was determined by spiking various levels of Acylamino-acid-releasing enzyme into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
95 |
89-101 |
|
Plasma |
97 |
91-103 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acylamino-acid-releasing enzyme and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
87-97% |
94-103% |
101-111% |
116-125% |
|
EDTA plasma(n=5) |
91-101% |
95-104% |
92-102% |
98-107% |
|
heparin plasma(n=5) |
108-117%
|
80-88% |
105-116% |
104-114% |
General Annotation
Sub Unit:
Homotetramer.
Function:
This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
Subcellular Location:
Cytoplasm
Database link
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Identification of oxidized protein hydrolase of human erythrocytes as acylpeptide hydrolase."
[3].
"Crystallization and preliminary X-ray studies of human erythrocyte acylpeptide hydrolase."
[4].
"Acylpeptide hydrolase: inhibitors and some active site residues of the human enzyme."
[5].
"The gene from the short arm of chromosome 3, at D3F15S2, frequently deleted in renal cell carcinoma, encodes acylpeptide hydrolase."
[6].
"Genetic relationship between acylpeptide hydrolase and acylase, two hydrolytic enzymes with similar binding but different catalytic specificities."
[7].
"Meta-analysis identifies 29 additional ulcerative colitis risk loci, increasing the number of confirmed associations to 47."
[8].
"Association analysis of 3p21 with Crohn's disease in a New Zealand population."
[9].
"Acyl peptide hydrolase, a serine proteinase isolated from conditioned medium of neuroblastoma cells, degrades the amyloid-beta peptide."
[10].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
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