Home > ELISA Kit > Rat Aco2 ELISA Kit

Aco2 (GeneName), Aconitate hydratase, mitochondrial (ProteinName), ACON_RAT.

Product Name:

Rat Aco2/ Aconitate hydratase, mitochondrial ELISA Kit

Cat.#:

E1263r

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

Aconitase, Citrate hydro-lyase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.625-40ng/mL

Sensitivity:

0.321ng/mL

Specificity:

Natural and recombinant rat Aconitate hydratase, mitochondrial

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Rat Aco2 ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations
Publication
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery

Recovery was determined by spiking various levels of Aconitate hydratase, mitochondrial into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	98	92-104
Plasma	100	94-106

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aconitate hydratase, mitochondrial and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	105-114%	82-91%	101-113%	97-106%
EDTA plasma(n=5)	104-116%	84-95%	109-118%	106-115%
heparin plasma(n=5)	97-109%	96-106%	108-119%	95-95%

General Annotation

Sub Unit:

Monomer.

Function:

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

Subcellular Location:

Mitochondrion

Database link

UniGene:

Rn.43737

SMR:

Q9ER34

STRING:

10116.ENSRNOP00000029144

KEGG:

rno:79250

Pfam:

PF00330

Uniprot:

Q9ER34

Related Product

Human ACO2 ELISA Kit

Mouse Aco2 ELISA Kit

Bovine ACO2 ELISA Kit

Pig ACO2 ELISA Kit

Human ACO2 CLIA Kit

Sequence length:

780

Sequence:

50:

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780:

TILLNHTFNE | TQIEWFRAGS | ALNRMKELQQ |

3D Structure:

Predicted Epitope:

Valid Sequence:

This product has not yet been referenced specifically in any publications.

[1].

"The DNA sequence of human chromosome 22."

(PMID: 10591208)

[2].

"Characterization of the human mitochondrial aconitase gene (ACO2)."

(PMID: 9630632)

[3].

"Mutations in the tricarboxylic acid cycle enzyme, aconitase 2, cause either isolated or syndromic optic neuropathy with encephalopathy and cerebellar atrophy."

(PMID: 25351951)

[4].

"Infantile cerebellar-retinal degeneration associated with a mutation in mitochondrial aconitase, ACO2."

(PMID: 22405087)

[5].

"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

(PMID: 20877624)

[6].

"A genome annotation-driven approach to cloning the human ORFeome."

(PMID: 15461802)

[7].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."