Aco2 (GeneName), Aconitate hydratase, mitochondrial (ProteinName), ACON_RAT.
Product Name:
Rat Aco2/ Aconitate hydratase, mitochondrial ELISA Kit
Cat.#:
E1263r
Brand:
EIAab®
Regulatory Status:
Alternative:
Aconitase, Citrate hydro-lyase
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.625-40ng/mL
Sensitivity:
0.321ng/mL
Specificity:
Natural and recombinant rat Aconitate hydratase, mitochondrial
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
-
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.9%
Recovery
Recovery was determined by spiking various levels of Aconitate hydratase, mitochondrial into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
98 |
92-104 |
Plasma |
100 |
94-106 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aconitate hydratase, mitochondrial and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
105-114% |
82-91% |
101-113% |
97-106% |
EDTA plasma(n=5) |
104-116% |
84-95% |
109-118% |
106-115% |
heparin plasma(n=5) |
97-109%
|
96-106% |
108-119% |
95-95% |
General Annotation
Sub Unit:
Monomer.
Function:
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Subcellular Location:
Mitochondrion
This product has not yet been referenced specifically in any publications.
[3].
"Mutations in the tricarboxylic acid cycle enzyme, aconitase 2, cause either isolated or syndromic optic neuropathy with encephalopathy and cerebellar atrophy."
[4].
"Infantile cerebellar-retinal degeneration associated with a mutation in mitochondrial aconitase, ACO2."
[5].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."
[7].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
[9].
"RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination."
[10].
"A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling."
Feedback Wall
Displaying result : 0
All customer
All customer
Default sort
Default sort
Newest first
Oldest first
No feedback yet, let's comment.






Message
Product Name *
Email *
Message: *
Inform
Size
Quantity
Price ($)
Subtotal 1 ($)
Subtotal 2:
$

Size
Quantity
Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:
Fax:
Email:sales@eiaab.com
Please contact us:
Tel:
Fax:
Email:sales@eiaab.com