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SCD (GeneName), Acyl-CoA desaturase (ProteinName), ACOD_HUMAN.
Product Name:

Human SCD/ Acyl-CoA desaturase ELISA Kit

Cat.#:

E9988h

Brand:
EIAab®
Regulatory Status:
Alternative:

Delta(9)-desaturase, Delta-9 desaturase, Fatty acid desaturase, Stearoyl-CoA desaturase, hSCD1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant human Acyl-CoA desaturase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human SCD ELISA Kit
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Human SCD ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.0%

Recovery
Recovery was determined by spiking various levels of Acyl-CoA desaturase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

102

96-108

Plasma

104

98-110

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acyl-CoA desaturase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

85-97%

102-112%

92-102%

106-116%

EDTA plasma(n=5)

98-107%

108-117%

98-108%

102-112%

heparin plasma(n=5)

88-98%

 

83-93%

89-98%

114-123%

 

General Annotation


Sub Unit:
May self-associate and form homodimers.


Function:
Stearyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates (PubMed:15907797, PubMed:18765284). Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA (PubMed:15907797, PubMed:18765284). Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids (PubMed:15610069). Plays an important role in lipid biosynthesis. Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation (By similarity). Plays an important role in body energy homeostasis (By similarity). Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides.


Subcellular Location:
Endoplasmic reticulum membrane Multi-pass membrane protein


This product has not yet been referenced specifically in any publications.
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