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Aco1 (GeneName), Cytoplasmic aconitate hydratase (ProteinName), ACOC_RAT.
Product Name:

Rat Aco1/ Cytoplasmic aconitate hydratase ELISA Kit

Cat.#:

E14200r

Brand:
EIAab®
Regulatory Status:
Alternative:

Aconitase, Citrate hydro-lyase, Iron regulatory protein 1, IRP1, Iron-responsive element-binding protein 1, IRE-BP 1, Ireb1, Irebp

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
15.6-1000U/L
Sensitivity:
7.2U/L
Specificity:
Natural and recombinant rat Cytoplasmic aconitate hydratase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Rat Aco1 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytoplasmic aconitate hydratase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

102-112%

107-116%

98-107%

84-94%

EDTA plasma(n=5)

97-106%

104-117%

97-107%

104-114%

heparin plasma(n=5)

104-114%

 

92-102%

101-112%

99-109%

 

General Annotation


Sub Unit:
Interacts (when associated with the 4Fe-4S) with FBXL5.


Function:
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Cloning of the cDNA encoding an RNA regulatory protein--the human iron-responsive element-binding protein."

[2].
"Crystal structure of human iron regulatory protein 1 as cytosolic aconitase."

[3].
"Homology between IRE-BP, a regulatory RNA-binding protein, aconitase, and isopropylmalate isomerase."

[4].
"Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and maturation of different subsets of cytosolic-nuclear iron-sulfur proteins."

[5].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

[6].
"Molecular control of the cytosolic aconitase/IRP1 switch by extramitochondrial frataxin."

[7].
"Associations of 9p21 variants with cutaneous malignant melanoma, nevi, and pigmentation phenotypes in melanoma-prone families with and without CDKN2A mutations."

[8].
"Control of iron homeostasis by an iron-regulated ubiquitin ligase."

[9].
"An E3 ligase possessing an iron-responsive hemerythrin domain is a regulator of iron homeostasis."

[10].
"Effect of hypoxia on the binding and subcellular distribution of iron regulatory proteins."
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