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Home  >  ELISA Kit  >  Human CHRM3 ELISA Kit
CHRM3 (GeneName), Muscarinic acetylcholine receptor M3 (ProteinName), ACM3_HUMAN.
Product Name:

Human CHRM3/ Muscarinic acetylcholine receptor M3 ELISA Kit

Cat.#:

E1838h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.23ng/mL
Specificity:
Natural and recombinant human Muscarinic acetylcholine receptor M3
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Neurosciences
Human CHRM3 ELISA Kit
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Human CHRM3 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.9%

Recovery
Recovery was determined by spiking various levels of Muscarinic acetylcholine receptor M3 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

95

89-101

Plasma

97

91-103

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Muscarinic acetylcholine receptor M3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

93-103%

102-112%

106-115%

89-99%

EDTA plasma(n=5)

89-98%

106-116%

101-110%

100-109%

heparin plasma(n=5)

96-96%

 

108-120%

101-114%

106-114%

 

General Annotation


Sub Unit:
Homodimer; the dimers can form tetramers (PubMed:23521066). Interacts with NALCN.


Function:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.


Subcellular Location:
Cell membrane Multi-pass membrane protein Cell junction Synapse Postsynaptic cell membrane Multi-pass membrane protein Basolateral cell membrane Multi-pass membrane protein


This product has not yet been referenced specifically in any publications.

[1].
"Muscarinic M3 receptor stimulation increases cigarette smoke-induced IL-8 secretion by human airway smooth muscle cells."

[2].
"Sequence variation in the promoter region of the cholinergic receptor muscarinic 3 gene and asthma and atopy."

[3].
"The muscarinic M3 acetylcholine receptor exists as two differently sized complexes at the plasma membrane."

[4].
"Family-based analysis of genetic variation underlying psychosis-inducing effects of cannabis: sibling analysis and proband follow-up."

[5].
"Muscarinic Acetylcholine Receptor M3 Mutation Causes Urinary Bladder Disease and a Prune-Belly-like Syndrome."
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