Acly (GeneName), ATP-citrate synthase (ProteinName), ACLY_MOUSE.
Product Name:
Mouse Acly/ ATP-citrate synthase ELISA Kit
Cat.#:
E14203m
Brand:
EIAab®
Regulatory Status:
Alternative:
ATP-citrate (pro-S-)-lyase, Citrate cleavage enzyme
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.174ng/mL
Specificity:
Natural and recombinant mouse ATP-citrate synthase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cardiovascular
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.6%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
Recovery
Recovery was determined by spiking various levels of ATP-citrate synthase into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
89 |
83-95 |
|
Plasma |
91 |
85-97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATP-citrate synthase and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
99-109% |
95-105% |
82-92% |
100-110% |
|
EDTA plasma(n=5) |
101-112% |
101-110% |
102-112% |
94-103% |
|
heparin plasma(n=5) |
108-119%
|
93-103% |
108-118% |
93-101% |
General Annotation
Sub Unit:
Homotetramer.
Function:
ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
Subcellular Location:
Cytoplasm
Database link
UniGene:
SMR:
STRING:
KEGG:
Pfam:
Uniprot:
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[2].
"Localization of the gene for ATP citrate lyase (ACLY) distal to gastrin(GAS) and proximal to D17S856 on chromosome 17q12-q21."
[3].
"Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth."
[5].
"Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas."
[6].
"Identification of the citrate-binding site of human ATP-citrate lyase using X-ray crystallography."
[7].
"Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study."
[8].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."
[9].
"Decreased levels of metabolic enzymes in pancreatic islets of patients with type 2 diabetes."
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