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Acly (GeneName), ATP-citrate synthase (ProteinName), ACLY_MOUSE.
Product Name:

Mouse Acly/ ATP-citrate synthase ELISA Kit

Cat.#:

E14203m

Brand:
EIAab®
Regulatory Status:
Alternative:

ATP-citrate (pro-S-)-lyase, Citrate cleavage enzyme

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.174ng/mL
Specificity:
Natural and recombinant mouse ATP-citrate synthase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cardiovascular
Mouse Acly ELISA Kit
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Mouse Acly ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Recovery
Recovery was determined by spiking various levels of ATP-citrate synthase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

89

83-95

Plasma

91

85-97

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATP-citrate synthase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-109%

95-105%

82-92%

100-110%

EDTA plasma(n=5)

101-112%

101-110%

102-112%

94-103%

heparin plasma(n=5)

108-119%

 

93-103%

108-118%

93-101%

 

General Annotation


Sub Unit:
Homotetramer.


Function:
ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Cloning and expression of a human ATP-citrate lyase cDNA."

[2].
"Localization of the gene for ATP citrate lyase (ACLY) distal to gastrin(GAS) and proximal to D17S856 on chromosome 17q12-q21."

[3].
"Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth."

[4].
"ADP-Mg2+ bound to the ATP-grasp domain of ATP-citrate lyase."

[5].
"Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas."

[6].
"Identification of the citrate-binding site of human ATP-citrate lyase using X-ray crystallography."

[7].
"Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study."

[8].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

[9].
"Decreased levels of metabolic enzymes in pancreatic islets of patients with type 2 diabetes."

[10].
"ATP-citrate lyase links cellular metabolism to histone acetylation."
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