首页 > 酶联免疫试剂盒 > Bovine ACTL6B ELISA Kit

ACTL6B (基因名), Actin-like protein 6B (蛋白名), ACL6B_BOVIN.

产品名称:

Bovine ACTL6B/ Actin-like protein 6B ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

Actin-related protein Baf53b, ArpNalpha, BRG1-associated factor 53B, BAF53B, ACTL6, BAF53B

检测方法:

ELISA

特异性:

Natural and recombinant bovine Actin-like protein 6B

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

Neurosciences

规格 & 价格: 登录.

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Bovine ACTL6B ELISA Kit

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Component of the bBAF complex, which includes at least actin (ACTB), ARID1A/BAF250, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCC2/BAF170, SMARCD2/BAF60B and SMARCE1/BAF57. Note that the bBAF complex is polymorphic in regard to the ATPase, SMARCA2 and SMARCA4 occupying mutually exclusive positions. PBRM1 does not belong to bBAF complex. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin.

功能:

Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth.

亚细胞位置:

Nucleus

数据库链接

UniGene:

Bt.8753

SMR:

A4FUX8

STRING:

9913.ENSBTAP00000010915

KEGG:

bta:504476

Pfam:

PF00022

Uniprot:

A4FUX8

相关产品

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Human ACE ELISA Kit

Human GDF5 ELISA Kit

序列长度:

426

序列:

50:

100:

150:

200:

250:

300:

350:

400:

426:

GTFQQMWISK | QEYEEGGKQC | VERKCP

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Large-scale sequencing of two regions in human chromosome 7q22: analysis of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17 genes."

(PMID: 9799793)

[2].

"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

(PMID: 20628086)

[3].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

(PMID: 15489334)

[4].

"Human chromosome 7: DNA sequence and biology."

(PMID: 12690205)

[5].

"The DNA sequence of human chromosome 7."

(PMID: 12853948)

[6].

"Identification of a polymorphic, neuron-specific chromatin remodeling complex."

(PMID: 12368262)

[7].

"Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues."

(PMID: 10380635)

[8].

"Architecture of the human interactome defines protein communities and disease networks."

(PMID: 28514442)

[9].

"Merkel cell polyomavirus recruits MYCL to the EP400 complex to promote oncogenesis."

(PMID: 29028833)

[10].

"Spatial Clustering of de Novo Missense Mutations Identifies Candidate Neurodevelopmental Disorder-Associated Genes."

(PMID: 28867141)

[1].

牛ACTL6BELISA试剂盒可以做多少个样本？

牛ACTL6BELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛ACTL6BELISA试剂盒使用视频？

牛ACTL6BELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛ACTL6BELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛ACTL6BELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛ACTL6BELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛ACTL6BELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛ACTL6BELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛ACTL6BELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛ACTL6BELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛ACTL6BELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛ACTL6BELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛ACTL6BELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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