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ACKR4 (基因名), Atypical chemokine receptor 4 (蛋白名), ACKR4_BOVIN.

产品名称:

Bovine ACKR4/ Atypical chemokine receptor 4 ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

C-C chemokine receptor type 11, C-C CKR-11, CC chemokine receptor-like 1, Protein PPR1, Putative gustatory receptor type B, CCR11, CCRL1

检测方法:

ELISA

特异性:

Natural and recombinant bovine Atypical chemokine receptor 4

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格: 登录.

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Forms heteromers with CXCR3. Interacts with ARRB1 and ARRB2.

功能:

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25. Chemokine-binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization. Plays an important role in controlling the migration of immune and cancer cells that express chemokine receptors CCR7 and CCR9, by reducing the availability of CCL19, CCL21, and CCL25 through internalization. Negatively regulates CXCR3-induced chemotaxis. Regulates T-cell development in the thymus.

亚细胞位置:

Early endosome Recycling endosome Cell membrane Multi-pass membrane protein Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via caveolae. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane (By similarity).

数据库链接

UniGene:

Bt.565

SMR:

P35350

STRING:

9913.ENSBTAP00000026082

KEGG:

bta:281672

Pfam:

PF00001

Uniprot:

P35350

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序列长度:

350

序列:

50:

100:

150:

200:

250:

300:

350:

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Characterization of mouse CCX-CKR, a receptor for the lymphocyte-attracting chemokines TECK/mCCL25, SLC/mCCL21 and MIP-3beta/mCCL19: comparison to human CCX-CKR."

(PMID: 11981810)

[2].

"Cutting edge: identification of a novel chemokine receptor that binds dendritic cell- and T cell-active chemokines including ELC, SLC, and TECK."

(PMID: 10706668)

[3].

"Cloning of CCRL1, an orphan seven transmembrane receptor related to chemokine receptors, expressed abundantly in the heart."

(PMID: 10767544)

[4].

"Inhibition of CXCR3-mediated chemotaxis by the human chemokine receptor-like protein CCX-CKR."

(PMID: 23121557)

[5].

"β-Arrestin recruitment and G protein signaling by the atypical human chemokine decoy receptor CCX-CKR."

(PMID: 23341447)

[6].

"The chemokine receptor CCX-CKR mediates effective scavenging of CCL19 in vitro."

(PMID: 16791897)

[7].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

(PMID: 15489334)

[8].

"CCR11 is a functional receptor for the monocyte chemoattractant protein family of chemokines."

(PMID: 10734104)

[9].

"[AH8165, a new muscle relaxant]."

(PMID: 16148)

[10].

"Endogenous expression of the atypical chemokine receptor CCX-CKR (CCRL1) gene in human embryonic kidney (HEK 293) cells."

(PMID: 26699909)

[1].

牛ACKR4ELISA试剂盒可以做多少个样本？

牛ACKR4ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛ACKR4ELISA试剂盒使用视频？

牛ACKR4ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛ACKR4ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛ACKR4ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛ACKR4ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛ACKR4ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛ACKR4ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛ACKR4ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛ACKR4ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛ACKR4ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛ACKR4ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛ACKR4ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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