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ACKR3 (基因名), Atypical chemokine receptor 3 (蛋白名), ACKR3_CANFA.

产品名称:

Dog ACKR3/ Atypical chemokine receptor 3 ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

C-X-C chemokine receptor type 7, CXC-R7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1, RDC-1, CMKOR1, CXCR7, RDC1

检测方法:

ELISA

特异性:

Natural and recombinant dog Atypical chemokine receptor 3

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格: 登录.

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Homodimer. Can form heterodimers with CXCR4; heterodimerization may regulate CXCR4 signaling activity (By similarity). Interacts with ARRB1 and ARRB2.

功能:

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1. Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development.

亚细胞位置:

Cell membrane Multi-pass membrane protein Cytoplasm Perinuclear region Early endosome Recycling endosome Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via clathrin-coated pits in a beta-arrestin-dependent manner. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane.

数据库链接

Uniprot:

P11613

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序列长度:

362

序列:

50:

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362:

ETEYSALEQN | AK

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"The chemokine SDF-1/CXCL12 binds to and signals through the orphan receptor RDC1 in T lymphocytes."

(PMID: 16107333)

[2].

"Expression and regulation of the orphan receptor RDC1 and its putative ligand in human dendritic and B cells."

(PMID: 16455976)

[3].

"Ubiquitination of CXCR7 controls receptor trafficking."

(PMID: 22457824)

[4].

"Carboxy-terminus of CXCR7 regulates receptor localization and function."

(PMID: 22300987)

[5].

"CXCR7 protein expression in human adult brain and differentiated neurons."

(PMID: 21655198)

[6].

"CXCR7: a new SDF-1-binding receptor in contrast to normal CD34(+) progenitors is functional and is expressed at higher level in human malignant hematopoietic cells."

(PMID: 20887389)

[7].

"The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects."

(PMID: 20388803)

[8].

"CXCR7 functions as a scavenger for CXCL12 and CXCL11."

(PMID: 20161793)

[9].

"Beta-arrestin- but not G protein-mediated signaling by the "decoy" receptor CXCR7."

(PMID: 20018651)

[10].

"Elucidation of CXCR7-mediated signaling events and inhibition of CXCR4-mediated tumor cell transendothelial migration by CXCR7 ligands."

(PMID: 19641136)

[1].

狗ACKR3ELISA试剂盒可以做多少个样本？

狗ACKR3ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

狗ACKR3ELISA试剂盒使用视频？

狗ACKR3ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

狗ACKR3ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的狗ACKR3ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

狗ACKR3ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

狗ACKR3ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析狗ACKR3ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

狗ACKR3ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集狗ACKR3ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

狗ACKR3ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

狗ACKR3ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

狗ACKR3ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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