ACKR2 (基因名), Atypical chemokine receptor 2 (蛋白名), ACKR2_HUMAN.
产品名称:
Human ACKR2/ Atypical chemokine receptor 2 ELISA Kit
非典型趋化因子受体2
货号:
E14064h
商标:
EIAab®
监管等级:
别名:
C-C chemokine receptor D6, Chemokine receptor CCR-10, Chemokine receptor CCR-9, Chemokine-binding protein 2, Chemokine-binding protein D6, CCBP2, CCR10, CMKBR9, D6
检测方法:
ELISA
实验类型:
Sandwich
检测范围:
31.2-2000pg/mL
灵敏度:
9.5pg/mL
特异性:
Natural and recombinant human Atypical chemokine receptor 2
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
登录.
实验步骤:
研究领域:
-
通用注释
亚单元:
N/A
功能:
Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling pathway. Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. Plays a major role in the immune silencing of macrophages during the resolution of inflammation. Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs.
亚细胞位置:
Early endosome
Recycling endosome
Cell membrane
Multi-pass membrane protein
Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via clathrin-coated pits. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane.
数据库链接
验证序列:
该产品尚未在任何出版物中被引用。
[1].
"The beta-chemokine receptor D6 is expressed by lymphatic endothelium and a subset of vascular tumors."
[2].
"Cloning, expression, and chromosomal mapping of a novel human CC-chemokine receptor (CCR10) that displays high-affinity binding for MCP-1 and MCP-3."
[3].
"Cloning and characterization of a novel promiscuous human beta-chemokine receptor D6."
[4].
"Review: Structure-function and biological properties of the atypical chemokine receptor D6."
[5].
"An analysis of the function and expression of D6 on lymphatic endothelial cells."
[6].
"β-arrestin-dependent activation of the cofilin pathway is required for the scavenging activity of the atypical chemokine receptor D6."
[7].
"Genetic variations of the chemokine scavenger receptor D6 are associated with liver inflammation in chronic hepatitis C."
[10].
"The promiscuous CC chemokine receptor D6 is a functional coreceptor for primary isolates of human immunodeficiency virus type 1 (HIV-1) and HIV-2 on astrocytes."
[1].
人非典型趋化因子受体2(ACKR2)ELISA试剂盒可以做多少个样本？
人非典型趋化因子受体2(ACKR2)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。
[2].
人非典型趋化因子受体2(ACKR2)ELISA试剂盒使用视频？
人非典型趋化因子受体2(ACKR2)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html
https://www.eiaab.com.cn/lesson-tech/805.html
[3].
人非典型趋化因子受体2(ACKR2)ELISA试剂盒是放在-20℃冰箱保存吗？
EIAab的人非典型趋化因子受体2(ACKR2)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。
[4].
人非典型趋化因子受体2(ACKR2)ELISA试剂盒原理？
双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
[5].
人非典型趋化因子受体2(ACKR2)ELISA试剂盒中需要使用的样品量是多少？
夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。
[6].
如何分析人非典型趋化因子受体2(ACKR2)ELISA试剂盒数据？
建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/
https://www.eiaab.com.cn/news/502/
[7].
人非典型趋化因子受体2(ACKR2)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？
除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。
[8].
收集人非典型趋化因子受体2(ACKR2)ELISA试剂盒血浆样本，用什么作为抗凝剂？
一般建议用EDTA和肝素作为抗凝剂。
[9].
人非典型趋化因子受体2(ACKR2)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？
人非典型趋化因子受体2(ACKR2)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。
[10].
人非典型趋化因子受体2(ACKR2)ELISA试剂盒样本如何保存？
尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。
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