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Acin1 (基因名), Apoptotic chromatin condensation inducer in the nucleus (蛋白名), ACINU_MOUSE.

产品名称:

Mouse Acin1/ Apoptotic chromatin condensation inducer in the nucleus ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

Acinus, Acinus

检测方法:

ELISA

特异性:

Natural and recombinant mouse Apoptotic chromatin condensation inducer in the nucleus

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

Cancer

规格 & 价格: 登录.

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) complexes consisting of RNPS1, SAP18 and different isoforms of ACIN1; the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Interacts with API5. Interacts with SRPK2 in a phosphorylation-dependent manner.

功能:

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.

亚细胞位置:

Nucleus Nucleus speckle Nucleus Nucleoplasm Phosphorylation on Ser-1179 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

数据库链接

UniGene:

Mm.297078

STRING:

10090.ENSMUSP00000022793

KEGG:

mmu:56215

Pfam:

PF16294

Uniprot:

Q9JIX8

相关产品

Human C19orf80 ELISA Kit

Human ASPRO ELISA Kit

Mouse Trap1a ELISA Kit

Human ACE ELISA Kit

Human GDF5 ELISA Kit

序列长度:

1338

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RERERDRDRG | RERDRRDTKR | HSRSRSRSTP | VRDRGGRR

3D 模型:

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免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation."

(PMID: 10490026)

[2].

"The antiapoptotic protein AAC-11 interacts with and regulates Acinus-mediated DNA fragmentation."

(PMID: 19387494)

[3].

"Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core."

(PMID: 16314458)

[4].

"ASAP, a novel protein complex involved in RNA processing and apoptosis."

(PMID: 12665594)

[5].

"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."

(PMID: 9734811)

[6].

"The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex."

(PMID: 22388736)

[7].

"Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study."

(PMID: 20800603)

[8].

"Human SAP18 mediates assembly of a splicing regulatory multiprotein complex via its ubiquitin-like fold."

(PMID: 20966198)

[9].

"NGF inhibits human leukemia proliferation by downregulating cyclin A1 expression through promoting acinus/CtBP2 association."

(PMID: 19668232)

[10].

"A critical role for AKT activation in protecting cells from ionizing radiation-induced apoptosis and the regulation of acinus gene expression."

(PMID: 19615784)

[1].

小鼠Acin1ELISA试剂盒可以做多少个样本？

小鼠Acin1ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠Acin1ELISA试剂盒使用视频？

小鼠Acin1ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠Acin1ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠Acin1ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠Acin1ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠Acin1ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠Acin1ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠Acin1ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠Acin1ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠Acin1ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠Acin1ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠Acin1ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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