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Chrna9 (基因名), Neuronal acetylcholine receptor subunit alpha-9 (蛋白名), ACHA9_RAT.

产品名称:

Rat Chrna9/ Neuronal acetylcholine receptor subunit alpha-9 ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

Nicotinic acetylcholine receptor subunit alpha-9, NACHR alpha-9, Acra9

检测方法:

ELISA

特异性:

Natural and recombinant rat Neuronal acetylcholine receptor subunit alpha-9

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

Neurosciences

规格 & 价格: 登录.

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Rat Chrna9 ELISA Kit

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Can form homo- or heterooligomeric channels in conjunction with CHRNA10. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 heterooligomers. Interacts with the alpha-conotoxin RgIA (PubMed:25740413).

功能:

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding induces a conformation change that leads to the opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this leads to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion.

亚细胞位置:

Cell junction Synapse Postsynaptic cell membrane Multi-pass membrane protein Cell membrane Multi-pass membrane protein

数据库链接

UniGene:

Rn.54463

STRING:

10116.ENSRNOP00000003382

KEGG:

rno:65024

Pfam:

PF02931

Uniprot:

P43144

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序列长度:

479

序列:

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479:

VAKVIDRFFM | WIFFAMVFVM | TVLIIARAD

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Why do young women smoke? V. Role of direct and interactive effects of nicotinic cholinergic receptor gene variation on neurocognitive function."

(PMID: 17559419)

[2].

"A novel human nicotinic receptor subunit, alpha10, that confers functionality to the alpha9-subunit."

(PMID: 11752216)

[3].

"Chromosome location and characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) gene."

(PMID: 12697997)

[4].

"Crystal structures of free and antagonist-bound states of human α9 nicotinic receptor extracellular domain."

(PMID: 25282151)

[5].

"Molecular basis for the differential sensitivity of rat and human α9α10 nAChRs to α-conotoxin RgIA."

(PMID: 22774872)

[6].

"A large-scale candidate gene association study of age at menarche and age at natural menopause."

(PMID: 20734064)

[7].

"Pharmacogenetics of antipsychotic response in the CATIE trial: a candidate gene analysis."

(PMID: 19156168)

[8].

"Multiple distinct risk loci for nicotine dependence identified by dense coverage of the complete family of nicotinic receptor subunit (CHRN) genes."

(PMID: 19259974)

[9].

"No evidence for association between 19 cholinergic genes and bipolar disorder."

(PMID: 17373692)

[10].

"Why do young women smoke? I. Direct and interactive effects of environment, psychological characteristics and nicotinic cholinergic receptor genes."

(PMID: 16314871)

[1].

大鼠Chrna9ELISA试剂盒可以做多少个样本？

大鼠Chrna9ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠Chrna9ELISA试剂盒使用视频？

大鼠Chrna9ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠Chrna9ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠Chrna9ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠Chrna9ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠Chrna9ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠Chrna9ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠Chrna9ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠Chrna9ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠Chrna9ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠Chrna9ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠Chrna9ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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