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Home  >  ELISA Kit  >  Rat Ace ELISA Kit
Ace (GeneName), Angiotensin-converting enzyme (ProteinName), ACE_RAT.
Product Name:

Rat Ace/ Angiotensin-converting enzyme ELISA Kit

Cat.#:

E0004r

Brand:
EIAab®
Regulatory Status:
Alternative:

ACE, Dipeptidyl carboxypeptidase I, Kininase II, CD143, Dcp1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.157ng/mL
Specificity:
Natural and recombinant rat Angiotensin-converting enzyme
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Rat Ace ELISA Kit
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Rat Ace ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery
Recovery was determined by spiking various levels of Angiotensin-converting enzyme into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

100

94-106

Plasma

102

96-108

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin-converting enzyme and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

96-106%

98-108%

87-99%

87-97%

EDTA plasma(n=5)

87-96%

104-114%

100-110%

90-100%

heparin plasma(n=5)

99-109%

 

103-113%

97-107%

89-98%

 

General Annotation


Sub Unit:
N/A


Function:
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.


Subcellular Location:
Cell membrane Single-pass type I membrane protein Cytoplasm Detected in both cell membrane and cytoplasm in neurons.



[1].
"Structural details on the binding of antihypertensive drugs captopril and enalaprilat to human testicular angiotensin I-converting enzyme."

[2].
"Polymorphisms in NOS3, ACE and PAI-1 genes and risk of spontaneous recurrent miscarriage in the Gaza Strip."

[3].
"Endothelial nitric oxide synthase, angiotensin-converting enzyme and angiotensinogen gene polymorphisms in hypertensive disorders of pregnancy."

[4].
"Renin-angiotensin system and alpha-adducin gene polymorphisms and their relation to responses to antihypertensive drugs: results from the GENRES study."

[5].
"Genetic risk factors for arterial ischemic stroke in children: a possible MTHFR and eNOS gene-gene interplay?"

[6].
"Structural details on the binding of antihypertensive drugs captopril and enalaprilat to human testicular angiotensin I-converting enzyme."

[7].
"Polymorphisms in NOS3, ACE and PAI-1 genes and risk of spontaneous recurrent miscarriage in the Gaza Strip."

[8].
"Endothelial nitric oxide synthase, angiotensin-converting enzyme and angiotensinogen gene polymorphisms in hypertensive disorders of pregnancy."

[9].
"Renin-angiotensin system and alpha-adducin gene polymorphisms and their relation to responses to antihypertensive drugs: results from the GENRES study."

[10].
"Genetic risk factors for arterial ischemic stroke in children: a possible MTHFR and eNOS gene-gene interplay?"
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