Ace (GeneName), Angiotensin-converting enzyme (ProteinName), ACE_MOUSE.
Product Name:
Mouse Ace/ Angiotensin-converting enzyme ELISA Kit
Cat.#:
E0004m
Brand:
EIAab®
Regulatory Status:
Alternative:
ACE, Dipeptidyl carboxypeptidase I, Kininase II, CD143, Dcp1
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
39pg/mL
Specificity:
Natural and recombinant mouse Angiotensin-converting enzyme
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
-
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (1)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.1%
Recovery
Recovery was determined by spiking various levels of Angiotensin-converting enzyme into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
97 |
91-103 |
Plasma |
99 |
93-105 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin-converting enzyme and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
87-97% |
87-99% |
87-99% |
105-115% |
EDTA plasma(n=5) |
92-102% |
110-119% |
108-118% |
108-120% |
heparin plasma(n=5) |
104-114%
|
87-97% |
108-119% |
85-97% |
General Annotation
Sub Unit:
N/A
Function:
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.
Subcellular Location:
Cell membrane
Single-pass type I membrane protein
Cytoplasm
Detected in both cell membrane and cytoplasm in neurons.
This product has not yet been referenced specifically in any publications.
[1].
"Structural details on the binding of antihypertensive drugs captopril and enalaprilat to human testicular angiotensin I-converting enzyme."
[2].
"Polymorphisms in NOS3, ACE and PAI-1 genes and risk of spontaneous recurrent miscarriage in the Gaza Strip."
[3].
"Endothelial nitric oxide synthase, angiotensin-converting enzyme and angiotensinogen gene polymorphisms in hypertensive disorders of pregnancy."
[4].
"Renin-angiotensin system and alpha-adducin gene polymorphisms and their relation to responses to antihypertensive drugs: results from the GENRES study."
[5].
"Genetic risk factors for arterial ischemic stroke in children: a possible MTHFR and eNOS gene-gene interplay?"
[6].
"Structural details on the binding of antihypertensive drugs captopril and enalaprilat to human testicular angiotensin I-converting enzyme."
[7].
"Polymorphisms in NOS3, ACE and PAI-1 genes and risk of spontaneous recurrent miscarriage in the Gaza Strip."
[8].
"Endothelial nitric oxide synthase, angiotensin-converting enzyme and angiotensinogen gene polymorphisms in hypertensive disorders of pregnancy."
[9].
"Renin-angiotensin system and alpha-adducin gene polymorphisms and their relation to responses to antihypertensive drugs: results from the GENRES study."
[10].
"Genetic risk factors for arterial ischemic stroke in children: a possible MTHFR and eNOS gene-gene interplay?"
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