Home > ELISA Kit > Mouse Ace ELISA Kit

Ace (GeneName), Angiotensin-converting enzyme (ProteinName), ACE_MOUSE.

Product Name:

Mouse Ace/ Angiotensin-converting enzyme ELISA Kit

Cat.#:

E0004m

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

ACE, Dipeptidyl carboxypeptidase I, Kininase II, CD143, Dcp1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78-5000pg/mL

Sensitivity:

39pg/mL

Specificity:

Natural and recombinant mouse Angiotensin-converting enzyme

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Size & Price: Sign In.

Attention

Mouse Ace ELISA Kit

Email *

Message *

Please Sign In.

Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Publication
Sequence / 3D
Feedback Wall (1)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Recovery

Recovery was determined by spiking various levels of Angiotensin-converting enzyme into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	99	93-105

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin-converting enzyme and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-97%	87-99%	87-99%	105-115%
EDTA plasma(n=5)	92-102%	110-119%	108-118%	108-120%
heparin plasma(n=5)	104-114%	87-97%	108-119%	85-97%

General Annotation

Sub Unit:

N/A

Function:

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.

Subcellular Location:

Cell membrane Single-pass type I membrane protein Cytoplasm Detected in both cell membrane and cytoplasm in neurons.

Database link

UniGene:

Mm.754

SMR:

P09470

STRING:

10090.ENSMUSP00000001963

KEGG:

mmu:11421

Pfam:

PF01401

Uniprot:

P09470

Related Product

Human ACE ELISA Kit

Rat Ace ELISA Kit

Bovine ACE ELISA Kit

Chicken ACE ELISA Kit

Human ACE CLIA Kit

Sequence length:

1312

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

900:

950:

1000:

1050:

1100:

1150:

1200:

1250:

1300:

1312:

PQFGSEVELR | HS

3D Structure:

Predicted Epitope:

Valid Sequence:

This product has not yet been referenced specifically in any publications.

[1].

"Structural details on the binding of antihypertensive drugs captopril and enalaprilat to human testicular angiotensin I-converting enzyme."

(PMID: 15236580)

[2].

"Polymorphisms in NOS3, ACE and PAI-1 genes and risk of spontaneous recurrent miscarriage in the Gaza Strip."

(PMID: 20134171)

[3].

"Endothelial nitric oxide synthase, angiotensin-converting enzyme and angiotensinogen gene polymorphisms in hypertensive disorders of pregnancy."

(PMID: 20186148)

[4].

"Renin-angiotensin system and alpha-adducin gene polymorphisms and their relation to responses to antihypertensive drugs: results from the GENRES study."

(PMID: 19057513)

[5].

"Genetic risk factors for arterial ischemic stroke in children: a possible MTHFR and eNOS gene-gene interplay?"

(PMID: 19372095)

[6].

"Structural details on the binding of antihypertensive drugs captopril and enalaprilat to human testicular angiotensin I-converting enzyme."

(PMID: 15236580)

[7].

"Polymorphisms in NOS3, ACE and PAI-1 genes and risk of spontaneous recurrent miscarriage in the Gaza Strip."

(PMID: 20134171)

[8].

"Endothelial nitric oxide synthase, angiotensin-converting enzyme and angiotensinogen gene polymorphisms in hypertensive disorders of pregnancy."

(PMID: 20186148)

[9].

"Renin-angiotensin system and alpha-adducin gene polymorphisms and their relation to responses to antihypertensive drugs: results from the GENRES study."

(PMID: 19057513)

[10].

"Genetic risk factors for arterial ischemic stroke in children: a possible MTHFR and eNOS gene-gene interplay?"

(PMID: 19372095)

Feedback Wall

Displaying result : 1

All customer

liusyan

Default sort

Newest first

Oldest first

wahaha