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Ache (GeneName), Acetylcholinesterase (ProteinName), ACES_RAT.
Product Name:

Rat Ache/ Acetylcholinesterase ELISA Kit

Cat.#:

E1195r

Brand:
EIAab®
Regulatory Status:
Alternative:

AChE

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.625-40ng/mL
Sensitivity:
0.312ng/mL
Specificity:
Natural and recombinant rat Acetylcholinesterase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Neurosciences
Rat Ache ELISA Kit
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Rat Ache ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery
Recovery was determined by spiking various levels of Acetylcholinesterase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

103

97-109

Plasma

105

99-111

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acetylcholinesterase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

101-111%

106-115%

97-107%

85-94%

EDTA plasma(n=5)

88-98%

102-111%

94-105%

100-112%

heparin plasma(n=5)

93-104%

 

103-113%

95-103%

95-104%

 

General Annotation


Sub Unit:
Homotetramer; composed of disulfide-linked homodimers. Catalytic forms H (GPI-anchor dimer) and T (asymmetric collagen-tailed), which differ in their C-terminus, account for all types of known ACHE forms. Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers.


Function:
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.


Subcellular Location:
Isoform H Cell membrane Lipid-anchor GPI-anchor Extracellular side



[1].
"Variability of AChE, BChE, and ChAT genes in the late-onset form of Alzheimer's disease and relationships with response to treatment with Donepezil and Rivastigmine."

[2].
"Structures of recombinant native and E202Q mutant human acetylcholinesterase complexed with the snake-venom toxin fasciculin-II."

[3].
"Expression of three alternative acetylcholinesterase messenger RNAs in human tumor cell lines of different tissue origins."

[4].
"Mutagenesis of human acetylcholinesterase. Identification of residues involved in catalytic activity and in polypeptide folding."

[5].
"Transferability of type 2 diabetes implicated loci in multi-ethnic cohorts from Southeast Asia."

[6].
"Comprehensive copy number variant (CNV) analysis of neuronal pathways genes in psychiatric disorders identifies rare variants within patients."

[7].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

[8].
"Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?"

[9].
"Structural evidence that human acetylcholinesterase inhibited by tabun ages through O-dealkylation."

[10].
"Donepezil, a potent acetylcholinesterase inhibitor, induces caspase-dependent apoptosis in human promyelocytic leukemia HL-60 cells."
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