Home > ELISA Kit > Rat Ache ELISA Kit

Ache (GeneName), Acetylcholinesterase (ProteinName), ACES_RAT.

Product Name:

Rat Ache/ Acetylcholinesterase ELISA Kit

Cat.#:

E1195r

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

AChE

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.625-40ng/mL

Sensitivity:

0.312ng/mL

Specificity:

Natural and recombinant rat Acetylcholinesterase

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Neurosciences

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Rat Ache ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 1 )
Publication
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery

Recovery was determined by spiking various levels of Acetylcholinesterase into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	103	97-109
Plasma	105	99-111

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acetylcholinesterase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-111%	106-115%	97-107%	85-94%
EDTA plasma(n=5)	88-98%	102-111%	94-105%	100-112%
heparin plasma(n=5)	93-104%	103-113%	95-103%	95-104%

General Annotation

Sub Unit:

Homotetramer; composed of disulfide-linked homodimers. Catalytic forms H (GPI-anchor dimer) and T (asymmetric collagen-tailed), which differ in their C-terminus, account for all types of known ACHE forms. Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers.

Function:

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

Subcellular Location:

Isoform H Cell membrane Lipid-anchor GPI-anchor Extracellular side

Database link

UniGene:

Rn.105879

SMR:

P37136

KEGG:

rno:83817

Pfam:

PF08674

Uniprot:

P37136

Related Product

Human ACHE ELISA Kit

Mouse Ache ELISA Kit

Bovine ACHE ELISA Kit

Chicken ACHE ELISA Kit

Human ACHE CLIA Kit

Sequence length:

614

Sequence:

50:

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600:

614:

QFDHYSKQER | CSDL

3D Model:

3D Structure:

Predicted Epitope:

Valid Sequence:

[1].

"Neuroprotective effect of tanshinone IIA-incubated mesenchymal stem cells on Aβ25-35-induced neuroinflammation"

NQ Huang, YY Li, YJ Zhou, et al.

From: Behavioural Brain Research

[1].

"Variability of AChE, BChE, and ChAT genes in the late-onset form of Alzheimer's disease and relationships with response to treatment with Donepezil and Rivastigmine."

(PMID: 18780301)

[2].

"Structures of recombinant native and E202Q mutant human acetylcholinesterase complexed with the snake-venom toxin fasciculin-II."

(PMID: 11053835)

[3].

"Expression of three alternative acetylcholinesterase messenger RNAs in human tumor cell lines of different tissue origins."

(PMID: 8299725)

[4].

"Mutagenesis of human acetylcholinesterase. Identification of residues involved in catalytic activity and in polypeptide folding."

(PMID: 1517212)

[5].

"Transferability of type 2 diabetes implicated loci in multi-ethnic cohorts from Southeast Asia."

(PMID: 21490949)

[6].

"Comprehensive copy number variant (CNV) analysis of neuronal pathways genes in psychiatric disorders identifies rare variants within patients."

(PMID: 20398908)

[7].

"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

(PMID: 20628086)

[8].

"Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?"

(PMID: 20602615)

[9].

"Structural evidence that human acetylcholinesterase inhibited by tabun ages through O-dealkylation."

(PMID: 20408548)

[10].

"Donepezil, a potent acetylcholinesterase inhibitor, induces caspase-dependent apoptosis in human promyelocytic leukemia HL-60 cells."

(PMID: 20522977)

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