Ace2 (GeneName), Angiotensin-converting enzyme 2 (ProteinName), ACE2_RAT.
Product Name:
Rat Ace2/ Angiotensin-converting enzyme 2 ELISA Kit
Cat.#:
E1886r
Brand:
EIAab®
Regulatory Status:
Alternative:
ACE-related carboxypeptidase
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.091 ng/mL
Specificity:
Natural and recombinant rat Angiotensin-converting enzyme 2
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
- Data
- Citations ( 1 )
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤≤5.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤≤9.2%
Recovery
Recovery was determined by spiking various levels of Angiotensin-converting enzyme 2 into serum and plasma.
Sample Type | Average(%) | Recovery Range(%) |
Serum | 97 | 91-103 |
Plasma | 99 | 93-105 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin-converting enzyme 2 and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1：2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 111-120% | 106-115% | 99-109% | 94-104% |
EDTA plasma(n=5) | 105-114% | 88-100% | 106-116% | 91-103% |
heparin plasma(n=5) | 98-108%
| 86-98% | 97-108% | 94-107% |
General Annotation
Sub Unit:
Interacts with ITGB1 and the catalytically active form of TMPRSS2.
Function:
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.
Subcellular Location:
Cell membrane
Single-pass type I membrane protein
Cytoplasm
Detected in both cell membrane and cytoplasm in neurons.
Database link
UniGene:
SMR:
STRING:
KEGG:
Pfam:
Uniprot:
Valid Sequence:
[1].
Chun Xi Liu, Qin Hu, Yan Wang, Wei Zhang, Zhi Yong Ma, Jin Bo Feng, Rong Wang,Xu Ping Wang, Bo Dong, Fei Gao, Ming Xiang Zhang, and Yun Zhang
From:
Molecular Medicine
[1].
"A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9."
[2].
"[Relationship of angiotensin-converting enzyme 2 gene polymorphisms and vulnerability to coronary heart disease in patients with type 2 diabetes mellitus]."
[3].
"Polymorphisms of ACE2 gene are associated with essential hypertension and antihypertensive effects of Captopril in women."
[4].
"Association of angiotensin-converting enzyme 2 (ACE2) gene polymorphisms with parameters of left ventricular hypertrophy in men. Results of the MONICA Augsburg echocardiographic substudy."
[5].
"Association analysis of common variants of ELN, NOS2A, APOE and ACE2 to intracranial aneurysm."
[7].
"Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2."
[8].
"Identification of an alternative 5'-untranslated exon and new polymorphisms of angiotensin-converting enzyme 2 gene: lack of association with SARS in the Vietnamese population."
[9].
"Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the severe-acute respiratory syndrome-coronavirus (SARS-CoV) receptor, angiotensin-converting enzyme-2 (ACE2)."
[10].
"Polymorphisms in the gene encoding angiotensin I converting enzyme 2 and diabetic nephropathy."
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