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Home  >  ELISA Kit  >  Rat Ace2 ELISA Kit
Ace2 (GeneName), Angiotensin-converting enzyme 2 (ProteinName), ACE2_RAT.
Product Name:

Rat Ace2/ Angiotensin-converting enzyme 2 ELISA Kit

Cat.#:

E1886r

Brand:
EIAab®
Regulatory Status:
Alternative:

ACE-related carboxypeptidase

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.091 ng/mL
Specificity:
Natural and recombinant rat Angiotensin-converting enzyme 2
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Rat Ace2 ELISA Kit
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Rat Ace2 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤≤9.2%

Recovery
Recovery was determined by spiking various levels of Angiotensin-converting enzyme 2 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin-converting enzyme 2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

111-120%

106-115%

99-109%

94-104%

EDTA plasma(n=5)

105-114%

88-100%

106-116%

91-103%

heparin plasma(n=5)

98-108%

 

86-98%

97-108%

94-107%

 

General Annotation


Sub Unit:
Interacts with ITGB1 and the catalytically active form of TMPRSS2.


Function:
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.


Subcellular Location:
Cell membrane Single-pass type I membrane protein Cytoplasm Detected in both cell membrane and cytoplasm in neurons.



[1].
Chun Xi Liu, Qin Hu, Yan Wang, Wei Zhang, Zhi Yong Ma, Jin Bo Feng, Rong Wang,Xu Ping Wang, Bo Dong, Fei Gao, Ming Xiang Zhang, and Yun Zhang

[1].
"A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9."

[2].
"[Relationship of angiotensin-converting enzyme 2 gene polymorphisms and vulnerability to coronary heart disease in patients with type 2 diabetes mellitus]."

[3].
"Polymorphisms of ACE2 gene are associated with essential hypertension and antihypertensive effects of Captopril in women."

[4].
"Association of angiotensin-converting enzyme 2 (ACE2) gene polymorphisms with parameters of left ventricular hypertrophy in men. Results of the MONICA Augsburg echocardiographic substudy."

[5].
"Association analysis of common variants of ELN, NOS2A, APOE and ACE2 to intracranial aneurysm."

[6].
"Myocardial infarction increases ACE2 expression in rat and humans."

[7].
"Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2."

[8].
"Identification of an alternative 5'-untranslated exon and new polymorphisms of angiotensin-converting enzyme 2 gene: lack of association with SARS in the Vietnamese population."

[9].
"Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the severe-acute respiratory syndrome-coronavirus (SARS-CoV) receptor, angiotensin-converting enzyme-2 (ACE2)."

[10].
"Polymorphisms in the gene encoding angiotensin I converting enzyme 2 and diabetic nephropathy."
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