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Home  >  ELISA Kit  >  Human ACE2 ELISA Kit
ACE2 (GeneName), Angiotensin-converting enzyme 2 (ProteinName), ACE2_HUMAN.
Product Name:

Human ACE2/ Angiotensin-converting enzyme 2 ELISA Kit

Cat.#:

E1886h

Brand:
EIAab®
Regulatory Status:
Alternative:

ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15, UNQ868/PRO1885

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.34ng/mL
Specificity:
Natural and recombinant human Angiotensin-converting enzyme 2
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Development Biology
Human ACE2 ELISA Kit
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Human ACE2 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.7%

Recovery
Recovery was determined by spiking various levels of Angiotensin-converting enzyme 2 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

94

88-100

Plasma

96

90-102

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin-converting enzyme 2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-106%

103-113%

113-113%

108-116%

EDTA plasma(n=5)

81-89%

94-106%

85-93%

101-113%

heparin plasma(n=5)

116-125%

 

102-111%

84-93%

102-111%

 

General Annotation


Sub Unit:
(Microbial infection) Interacts with SARS coronavirus/SARS-CoV and human coronavirus NL63/HCoV-NL63 spike glycoprotein (PubMed:14647384, PubMed:15452268, PubMed:15791205, PubMed:15897467).


Function:
(Microbial infection) Acts as a receptor for SARS coronavirus/SARS-CoV and human coronavirus NL63/HCoV-NL63.


Subcellular Location:
Cell membrane Single-pass type I membrane protein Cytoplasm Detected in both cell membrane and cytoplasm in neurons.


This product has not yet been referenced specifically in any publications.

[1].
"A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9."

[2].
"[Relationship of angiotensin-converting enzyme 2 gene polymorphisms and vulnerability to coronary heart disease in patients with type 2 diabetes mellitus]."

[3].
"Polymorphisms of ACE2 gene are associated with essential hypertension and antihypertensive effects of Captopril in women."

[4].
"Association of angiotensin-converting enzyme 2 (ACE2) gene polymorphisms with parameters of left ventricular hypertrophy in men. Results of the MONICA Augsburg echocardiographic substudy."

[5].
"Association analysis of common variants of ELN, NOS2A, APOE and ACE2 to intracranial aneurysm."

[6].
"Myocardial infarction increases ACE2 expression in rat and humans."

[7].
"Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2."

[8].
"Identification of an alternative 5'-untranslated exon and new polymorphisms of angiotensin-converting enzyme 2 gene: lack of association with SARS in the Vietnamese population."

[9].
"Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the severe-acute respiratory syndrome-coronavirus (SARS-CoV) receptor, angiotensin-converting enzyme-2 (ACE2)."

[10].
"Polymorphisms in the gene encoding angiotensin I converting enzyme 2 and diabetic nephropathy."
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