首页 > 酶联免疫试剂盒 > Rat Acadsb ELISA Kit

Acadsb (基因名), Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (蛋白名), ACDSB_RAT.

产品名称:

Rat Acadsb/ Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial ELISA Kit
线粒体，短/支链特异性酰基辅酶a脱氢酶

货号:

E12556r

商标:

EIAab^®

监管等级:

RUO

别名:

SBCAD, 2-methyl branched chain acyl-CoA dehydrogenase, 2-MEBCAD, 2-methylbutyryl-coenzyme A dehydrogenase, 2-methylbutyryl-CoA dehydrogenase

检测方法:

ELISA

特异性:

Natural and recombinant rat Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

Cardiovascular

规格 & 价格: 登录.

关注

Rat Acadsb ELISA Kit

邮箱 *

消息 *

Please 登录.

产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

通用注释

亚单元:

Homotetramer.

功能:

Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent.

亚细胞位置:

Mitochondrion matrix

数据库链接

UniGene:

Rn.44423

SMR:

P70584

STRING:

10116.ENSRNOP00000027999

KEGG:

rno:25618

Pfam:

PF00441

Uniprot:

P70584

相关产品

Human ACADSB ELISA Kit

Mouse Acadsb ELISA Kit

Bovine ACADSB ELISA Kit

Human ACADSB CLIA Kit

Mouse Acadsb CLIA Kit

序列长度:

432

序列:

50:

100:

150:

200:

250:

300:

350:

400:

432:

EKFFRDAKIG | TIYEGTSNIQ | LNTIAKHIDA | EY

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Short/branched-chain acyl-CoA dehydrogenase deficiency due to an IVS3+3A>G mutation that causes exon skipping."

(PMID: 16317551)

[2].

"Isolated 2-methylbutyrylglycinuria caused by short/branched-chain acyl-CoA dehydrogenase deficiency: identification of a new enzyme defect, resolution of its molecular basis, and evidence for distinct acyl-CoA dehydrogenases in isoleucine and valine metabolism."

(PMID: 11013134)

[3].

"Localization of short/branched chain acyl-CoA dehydrogenase (ACADSB) to human chromosome 10."

(PMID: 7759115)

[4].

"Isolation and expression of a cDNA encoding the precursor for a novel member (ACADSB) of the acyl-CoA dehydrogenase gene family."

(PMID: 7698750)

[5].

"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

(PMID: 20877624)

[6].

"Association of genetic polymorphisms of ACADSB and COMT with human hypertension."

(PMID: 17143180)

[7].

"A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease."

(PMID: 16385451)

[8].

"Inborn errors of isoleucine degradation: a review."

(PMID: 16950638)

[9].

"2-ethylhydracrylic aciduria in short/branched-chain acyl-CoA dehydrogenase deficiency: application to diagnosis and implications for the R-pathway of isoleucine oxidation."

(PMID: 15615815)

[10].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

(PMID: 15489334)

[1].

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒可以做多少个样本？

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒使用视频？

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠线粒体，短/支链特异性酰基辅酶a脱氢酶(Acadsb)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

反馈墙

评论数 : 0

所有用户

默认排序

Rat Acadsb/ Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial ELISA Kit 线粒体，短/支链特异性酰基辅酶a脱氢酶

E12556r

SBCAD, 2-methyl branched chain acyl-CoA dehydrogenase, 2-MEBCAD, 2-methylbutyryl-coenzyme A dehydrogenase, 2-methylbutyryl-CoA dehydrogenase

Rat Acadsb/ Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial ELISA Kit
线粒体，短/支链特异性酰基辅酶a脱氢酶