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Acadm (GeneName), Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ProteinName), ACADM_RAT.
Product Name:

Rat Acadm/ Medium-chain specific acyl-CoA dehydrogenase, mitochondrial ELISA Kit

Cat.#:

E10433r

Brand:
EIAab®
Regulatory Status:
Alternative:

MCAD

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.78-50ng/mL
Sensitivity:
0.39ng/mL
Specificity:
Natural and recombinant rat Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cardiovascular
Rat Acadm ELISA Kit
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Rat Acadm ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.2%

Recovery
Recovery was determined by spiking various levels of Medium-chain specific acyl-CoA dehydrogenase, mitochondrial into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

91

85-97

Plasma

93

87-99

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Medium-chain specific acyl-CoA dehydrogenase, mitochondrial and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

88-97%

104-114%

108-116%

105-114%

EDTA plasma(n=5)

99-109%

90-99%

100-109%

94-104%

heparin plasma(n=5)

105-114%

 

108-117%

89-101%

105-116%

 

General Annotation


Sub Unit:
Homotetramer.


Function:
Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta-oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).


Subcellular Location:
Mitochondrion matrix


This product has not yet been referenced specifically in any publications.

[1].
"Medium-chain acyl-CoA dehydrogenase (MCAD) mutations identified by MS/MS-based prospective screening of newborns differ from those observed in patients with clinical symptoms: identification and characterization of a new, prevalent mutation that results in mild MCAD deficiency."

[2].
"A985G mutation incidence in the medium-chain acyl-CoA dehydrogenase (MCAD) gene in Brazil."

[3].
"A novel tandem mass spectrometry method for rapid confirmation of medium- and very long-chain acyl-CoA dehydrogenase deficiency in newborns."

[4].
"Medium-chain acyl-CoA dehydrogenase deficiency: genotype-biochemical phenotype correlations."

[5].
"Population spectrum of ACADM genotypes correlated to biochemical phenotypes in newborn screening for medium-chain acyl-CoA dehydrogenase deficiency."

[6].
"Newborns with C8-acylcarnitine level over the 90th centile have an increased frequency of the common MCAD 985A>G mutation."

[7].
"Blood acylcarnitine levels in normal newborns and heterozygotes for medium-chain acyl-CoA dehydrogenase deficiency: a relationship between genotype and biochemical phenotype?"

[8].
"Molecular and functional characterisation of mild MCAD deficiency."

[9].
"Evaluation of newborn screening for medium chain acyl-CoA dehydrogenase deficiency in 275 000 babies."

[10].
"Medium chain acyl-CoA dehydrogenase deficiency human genome epidemiology review."
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