ACADM (GeneName), Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ProteinName), ACADM_HUMAN.
Product Name:
Human ACADM/ Medium-chain specific acyl-CoA dehydrogenase, mitochondrial ELISA Kit
Cat.#:
E10433h
Brand:
EIAab®
Regulatory Status:
Alternative:
MCAD
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
40.1pg/mL
Specificity:
Natural and recombinant human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cardiovascular
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.6%
Recovery
Recovery was determined by spiking various levels of Medium-chain specific acyl-CoA dehydrogenase, mitochondrial into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
98 |
92-104 |
|
Plasma |
100 |
94-106 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Medium-chain specific acyl-CoA dehydrogenase, mitochondrial and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
103-115% |
107-119% |
87-97% |
96-108% |
|
EDTA plasma(n=5) |
105-116% |
87-97% |
96-108% |
116-116% |
|
heparin plasma(n=5) |
96-105%
|
80-90% |
105-115% |
92-101% |
General Annotation
Sub Unit:
Homotetramer. Interacts with the heterodimeric electron transfer flavoprotein ETF.
Function:
Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta-oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Subcellular Location:
Mitochondrion matrix
Database link
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Medium-chain acyl-CoA dehydrogenase (MCAD) mutations identified by MS/MS-based prospective screening of newborns differ from those observed in patients with clinical symptoms: identification and characterization of a new, prevalent mutation that results in mild MCAD deficiency."
[2].
"A985G mutation incidence in the medium-chain acyl-CoA dehydrogenase (MCAD) gene in Brazil."
[3].
"A novel tandem mass spectrometry method for rapid confirmation of medium- and very long-chain acyl-CoA dehydrogenase deficiency in newborns."
[4].
"Medium-chain acyl-CoA dehydrogenase deficiency: genotype-biochemical phenotype correlations."
[5].
"Population spectrum of ACADM genotypes correlated to biochemical phenotypes in newborn screening for medium-chain acyl-CoA dehydrogenase deficiency."
[6].
"Newborns with C8-acylcarnitine level over the 90th centile have an increased frequency of the common MCAD 985A>G mutation."
[7].
"Blood acylcarnitine levels in normal newborns and heterozygotes for medium-chain acyl-CoA dehydrogenase deficiency: a relationship between genotype and biochemical phenotype?"
[9].
"Evaluation of newborn screening for medium chain acyl-CoA dehydrogenase deficiency in 275 000 babies."
[10].
"Medium chain acyl-CoA dehydrogenase deficiency human genome epidemiology review."
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