027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Mouse Acaca ELISA Kit
Acaca (GeneName), Acetyl-CoA carboxylase 1 (ProteinName), ACACA_MOUSE.
Product Name:

Mouse Acaca/ Acetyl-CoA carboxylase 1 ELISA Kit

Cat.#:

E1284m

Brand:
EIAab®
Regulatory Status:
Alternative:

ACC1, ACC-alpha, Acetyl-CoA carboxylase 265, Acac, Gm738

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
31.2-2000pg/ml
Sensitivity:
15.72pg/mL
Specificity:
Natural and recombinant mouse Acetyl-CoA carboxylase 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Metabolism
Mouse Acaca ELISA Kit
Size & Price: Sign In.
×
Mouse Acaca ELISA Kit
Email *
Message *
Please Sign In.
Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.1%

Recovery
Recovery was determined by spiking various levels of Acetyl-CoA carboxylase 1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

85

80-91

Plasma

87

81-93

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acetyl-CoA carboxylase 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

104-113%

83-92%

101-110%

85-94%

EDTA plasma(n=5)

100-109%

102-111%

90-100%

101-110%

heparin plasma(n=5)

103-103%

 

87-97%

106-116%

107-116%

 

General Annotation


Sub Unit:
Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.


Function:
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Acetyl-CoA carboxylase alpha gene and breast cancer susceptibility."

[2].
"Acetyl-coenzyme A carboxylase alpha gene variations may be associated with the direct effects of some antipsychotics on triglyceride levels."

[3].
"Haplotype-based analysis of common variation in the acetyl-coA carboxylase alpha gene and breast cancer risk: a case-control study nested within the European Prospective Investigation into Cancer and Nutrition."

[4].
"BRCA1 affects lipid synthesis through its interaction with acetyl-CoA carboxylase."

[5].
"Asymmetric expression of transcripts derived from the shared promoter between the divergently oriented ACACA and TADA2L genes."

[6].
"Acetyl-CoA carboxylase alpha gene and breast cancer susceptibility."

[7].
"Acetyl-coenzyme A carboxylase alpha gene variations may be associated with the direct effects of some antipsychotics on triglyceride levels."

[8].
"Haplotype-based analysis of common variation in the acetyl-coA carboxylase alpha gene and breast cancer risk: a case-control study nested within the European Prospective Investigation into Cancer and Nutrition."

[9].
"BRCA1 affects lipid synthesis through its interaction with acetyl-CoA carboxylase."

[10].
"Asymmetric expression of transcripts derived from the shared promoter between the divergently oriented ACACA and TADA2L genes."
Close
Sample Data
Close
Sample Data
Close
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name *
Email *
Message: *
Inform
new Message
Size Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Size Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com