Acaca (GeneName), Acetyl-CoA carboxylase 1 (ProteinName), ACACA_MOUSE.
Product Name:
Mouse Acaca/ Acetyl-CoA carboxylase 1 ELISA Kit
Cat.#:
E1284m
Brand:
EIAab®
Regulatory Status:
Alternative:
ACC1, ACC-alpha, Acetyl-CoA carboxylase 265, Acac, Gm738
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
31.2-2000pg/ml
Sensitivity:
15.72pg/mL
Specificity:
Natural and recombinant mouse Acetyl-CoA carboxylase 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Metabolism
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤7.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤10.1%
Recovery
Recovery was determined by spiking various levels of Acetyl-CoA carboxylase 1 into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
85 |
80-91 |
|
Plasma |
87 |
81-93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acetyl-CoA carboxylase 1 and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
104-113% |
83-92% |
101-110% |
85-94% |
|
EDTA plasma(n=5) |
100-109% |
102-111% |
90-100% |
101-110% |
|
heparin plasma(n=5) |
103-103%
|
87-97% |
106-116% |
107-116% |
General Annotation
Sub Unit:
Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.
Function:
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Subcellular Location:
Cytoplasm
Database link
UniGene:
SMR:
STRING:
KEGG:
Pfam:
Uniprot:
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[2].
"Acetyl-coenzyme A carboxylase alpha gene variations may be associated with the direct effects of some antipsychotics on triglyceride levels."
[3].
"Haplotype-based analysis of common variation in the acetyl-coA carboxylase alpha gene and breast cancer risk: a case-control study nested within the European Prospective Investigation into Cancer and Nutrition."
[4].
"BRCA1 affects lipid synthesis through its interaction with acetyl-CoA carboxylase."
[5].
"Asymmetric expression of transcripts derived from the shared promoter between the divergently oriented ACACA and TADA2L genes."
[7].
"Acetyl-coenzyme A carboxylase alpha gene variations may be associated with the direct effects of some antipsychotics on triglyceride levels."
[8].
"Haplotype-based analysis of common variation in the acetyl-coA carboxylase alpha gene and breast cancer risk: a case-control study nested within the European Prospective Investigation into Cancer and Nutrition."
[9].
"BRCA1 affects lipid synthesis through its interaction with acetyl-CoA carboxylase."
[10].
"Asymmetric expression of transcripts derived from the shared promoter between the divergently oriented ACACA and TADA2L genes."
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